AIM: To evaluate the effect of Xueshuantong injection on lipopolysaccharide (IPS)-induced experimental disseminated intravascular coagulation (DIC) in rabbits. METHODS: To establish the LPS-induced DIC model , LPS was continuously injected into the rabbit autricular vein for 6 h. The survival rate of the rabbits was recorded after 24 h. The plasma levels of alanine aminotransfERαse (ALT) and blood urine nitrogen (BUN) were detected. Activated partial thromboplastin time (APTT) , prothrombin time (PT) , platelet count and fibrinogen concentration were measured. The activity of protein C, antithrombin III ( AT III ) and the concentration of tumor necrosis factor a (TNF-α) were also determined. RESULTS: The survival rate of DIC rabbits was only 26.7%. BUN and ALT increased obviously. APTT and PT became much longer, platelet count, fibrinogen, protein C and AT HI decreased obviously, and plasma TNF-α increased remarkably. The intravenous administration of Xueshuantong injection increased the survival rate of DIC rabbits up to 66. 7% in a dose-dependent manner. Xueshuantong injection also decreased the plasma levels of BUN, ALT, APTT, PT and TNF-α significantly, while increased the levels of fibrinogen, platelet, protein C and ATIII in plasma. CONCLUSION: Xueshuantong injection has thERαpeutic effect on LPS-induced DIC in rabbits.%目的:研究血栓通注射液对脂多糖(LPS)诱导的兔弥散性血管内凝血(DIC)的影响.方法:对兔耳缘静脉持续滴注LPS以建立兔DIC模型,记录各组动物在24h后的生存率；全自动血浆分析仪测定丙氨酸氨基转移酶(ALT)和血尿素氮(BUN)；用全自动凝血分析仪检测活化部分凝血活酶时间(APT)和凝血酶原时间(PT)；用凝固法测定纤维蛋白原含量；全自动血细胞分析仪进行血小板计数；发色底物法测定蛋白C和抗凝血酶Ⅲ的活性；ELISA法检测血浆中肿瘤坏死因子α(TNF-α)的含量.结果:持续从兔耳缘静脉滴注LPS后,DIC模型组动物大量死亡,ALT和BUN显著升高,APTT和PT显著延长,蛋白C和抗凝血酶Ⅲ的活性明显降低,血小板计数明显减少,血浆中TNF-α的含量显著升高.注射血栓通注射液后,动物的死亡率明显下降,ALT和BUN明显下降,APTT和PT明显缩短,血小板计数明显上升,纤维蛋白原含量显著提高,蛋白C和抗凝血酶Ⅲ活性明显提升；血浆TNF-α的含量明显降低.结论:血栓通注射液对LPS所诱导的兔DIC有良好的拮抗作用.
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