目的:研究口虾蛄提取物(extract of Oratosquilla,EOS)对人低分化鼻咽癌细胞株CNE-2的迁移及体外血管生成拟态的影响.方法:用不同浓度(0 mg/L、125 mg/L、250 mg/L、500 mg/L) EOS处理CNE-2细胞24h后,创伤修复实验检测细胞迁移能力；通过Matrigel三维细胞培养观察CNE-2细胞形成类血管网状结构的能力及其特点；体外管道形成抑制实验检测不同浓度EOS对CNE-2细胞管道形成能力的影响；Western blotting法检测不同浓度EOS对CNE-2细胞fascin 1和血管内皮生长因子(VEGF)蛋白表达的影响.结果:EOS可以显著降低CNE-2细胞的迁移能力,与对照组比较差异有统计学意义(P ＜0.01)；CNE-2细胞在Matrigel上培养能形成类似血管的网状样结构；EOS能以剂量依赖方式抑制CNE-2细胞体外管道形成的数量(P＜0.01)；EOS能抑制CNE-2细胞中fas-cin 1和VEGF蛋白的表达(P＜0.01),且其管状结构数量与2种蛋白变化趋势呈正相关(P＜0.05).结论:CNE-2细胞具有血管生成拟态的能力；EOS能够抑制CNE-2细胞的迁移和体外血管生成拟态的能力,其机制可能与下调fascin 1和VEGF蛋白的表达有关.%AIM:To study the inhibitory effect of the extract of Oratosquilla (EOS) on the migration and vasculogenic mimicry in human poorly differentiated nasopharyngeal carcinoma cell line CNE-2.METHODS:CNE-2 cells were cultured in the medium with different concentrations of EOS (0 mg/L,125 mg/L,250 mg/L and 500 mg/L).The migration of CNE-2 cells and the formation of tube-like structures (TLSs) by CNE-2 cells were determined with wound healing assay and in vitro anti-angiogenesis test,respectively.The formation of TLSs by CNE-2 cells and their structural characteristics were observed by anti-angiogenesis test on the Matrigel.The protein expression of fascin 1 and vascular endothelial growth factor (VEGF) was detected by Western blotting.RESULTS:Compared with control group,EOS significantly decreased the migration velocity of CNE-2 cells in a dose-dependent manner.CNE-2 cells formed TLSs on the Matrigel,and the formation of TLSs by CNE-2 cells was inhibited by EOS in a dose-dependent manner.The expression of fascin 1 and VEGF in CNE-2 cells was also decreased after treatment with EOS.A positive correlation between the expression of fascin 1/VEGF and the formation of TLSs by CNE-2 cells was observed.CONCLUSION:CNE-2 cells form TLSs on the Matrigel,and EOS inhibits the migration and vasculogenic mimicry of CNE-2 cells,which are related with down-regulating the expression of fascin 1 and VEGF in CNE-2 cells.
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