AIM:To explore the effect of high tumor necrosis factor α ( TNF - α ) level and pre - treatment of epigall o cat he chin - 3 gallate ( EGCG ) on the process of wound healing in dermal fibroblasts. METHODS: Primary dermal fibroblasts were cultured in vitro. The cells were treated with TNF -α at α concentration of 10μg/L for 24 h or co - treated with EGCG ( 40μmol/L). The cell counting assay was used to observe the proliferation. The cell migration was assessed by wound healing assay. Western blotting was used to observe the expression of collagen type I. RESULTS: High TNF - a level significantly inhibited the proliferation and migration of dermal fibroblasts. However, EGCG pre -treatment attenuated the inhibitory effect of TNF -α on the proliferation in a dose - dependent manner. The inhibited cell migration was also improved by EGCG. The expression of collagen type I was down - regulated by TNF -α and recovered by EGCG pre - treatment. CONCLUSION: EGCG abrogates the inhibitory effect of TNF - a on the proliferation and migration of dermal fibroblasts in wound healing. The expression of collagen type I is also improved. The results suggest that EGCG has protective effect on wound healing.%目的:探讨高浓度肿瘤坏死因子α(TNF-α)对人皮肤创伤愈合中成纤维细胞的抑制作用以及表没食子儿茶素没食子酸酯(EGCG)的干预效应.方法:体外培养原代人皮肤成纤维细胞,以10 μg/L TNF-α作用24 h或联合EGCG预处理干预,用细胞计数试剂盒观察细胞增殖,细胞划痕愈合实验观察成纤维细胞的迁移,Western blotting法研究I型胶原蛋白的表达.结果:TNF-α显著抑制皮肤成纤维细胞的增殖和迁移,EGCG呈浓度依赖性地改善TNF-α的增殖抑制作用,以EGCG(40 μmol/L)预处理后,TNF-α对细胞迁移的抑制作用也得到恢复.Western blotting发现TNF-α还能抑制I型胶原蛋白的表达,而加入EGCG(40 μmol/L)后其表达有显著恢复.结论:EGCG能显著改善高浓度TNF-α对人皮肤成纤维细胞增殖和迁移的抑制作用,并恢复I型胶原的表达,从而促进皮肤创口的愈合.
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