AIM: To investigate the role of arthralgia due to dampness in the pathogenesis of acute gouty arthritis and to observe the anti - arthralgic effects of Qushi - Chubi compound prescription of traditional Chinese medicines ( TCM ) on gouty arthritis in a rat model. METHODS: Forty SPF Wistar rats were randomly divided into 5 groups: blank group, model group, colchicine group, TCM low — dose group and TCM high — dose group. Monosodium urate crystals were injected into the rat ankle to induce acute gouty arthritis. The decoction of Qushi - Chubi compound prescription was given intragastrically for 7 days. Colchicine was used as a positive model drug in the model rats. The expression levels of inter-leukin — 1(3 ( IL — 1β), interleukin — 1 receptor ( IL — 1R ) and myeloid differentiation factor 88 ( MyD88 ) in the joint tissues were observed. RESULTS: Seven days after intragastrical administration, the degrees of joint swelling in colchicine group and TCM treatment groups were significantly lower than that in model group ( P < 0. 01 ), and that in TCM high -dose group was even lower than that in colchicine group with statistical significance. The index of inflammatory responses and joint dysfunction in model group was the highest and was higher than those in colchicine group and TCM treatment groups. The serum levels of IL - 1(3 and IL - 1R in TCM high - dose group were significantly lower than those in model group and colchicine group ( P <0. 05 ). CONCLUSION: The Qushi - Chubi compound prescription effectively inhibits the expression of inflammatory cytokines in rats with acute gouty arthritis, and also attenuates the inflammatory responses of the arthralgia joints.%目的:探讨祛湿除痹复方对大鼠急性痛风性关节炎的可能作用机制,验证急性痛风性关节炎的湿热痹阻病机.方法:将40只Wistar大鼠随机分5组,每组8只,即空白组、模型组、秋水仙碱组、中药低剂量组和中药高剂量组.采用尿酸单钠晶体溶液踝关节注射法建立急性痛风性关节炎模型.以祛湿除痹复方与秋水仙碱对比治疗,观察祛湿除痹复方对大鼠关节各种症状(炎症、肿胀度及活动障碍)及大鼠关节组织内白细胞介素1β(IL-1β)、白细胞介素1受体(IL-1R)和髓样分化因子88(MyD88)表达水平的影响.结果:灌胃给药7 d后秋水仙碱组和中药各剂量组与模型组比较,大鼠关节肿胀程度差异显著(P＜0.01),秋水仙碱组和中药高剂量组比较差异有统计学意义(P＜0.05).各组大鼠关节炎症指数及功能障碍指数积分比较,模型组积分最高.造模后8和72 h各组积分均降低(P＜0.05),模型组与各用药组比较差异显著 (P＜0.05).关节软组织内IL-1β、IL-1R及MyD88的平均吸光度以模型组为最高,中药低剂量组和秋水仙碱组比较无明显差异(P＞0.05),而与中药高剂量组比较有显著差异(P＜0.05).结论:祛湿除痹复方能有效抑制实验性急性痛风性关节炎大鼠关节组织内炎症因子的表达,从而改善模型大鼠各种关节症状.
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