目的:研究12-肉豆蔻酸-13-乙酸佛波酯(PMA)对K562细胞BCR/ABL与Fyn mRNA表达的影响及两者之间的关系.方法:1～250 μg/L PMA刺激K562细胞 24 h后,应用实时荧光定量PCR技术检测各组BCR/ABL和Fyn mRNA的水平.结果:PMA明显抑制BCR/ABL和Fyn mRNA的水平,该下调作用均具有显著的量效关系,且BCR/ABL和Fyn的mRNA表达水平下调表现出明显的相关性.比较刺激Molt-4细胞株的结果,PMA抑制作用具有细胞选择性.K562细胞株经诱导后出现伪足样的形态改变.结论:PMA通过抑制BCR/ABL融合基因的转录下调Fyn转录水平.%ADM: To study the effects of phorbol 12 — myristate 13 — acetate ( PMA ) on the mRNA expression of BCR/ABL and Fyn in K562 cells, and to explore their relationship. METHODS: The K562 cells were stimulated by PMA at a series of concentrations ( 1 ~ 250 μg/L ) for 24 h, and the mRNA expression levels of BCR/ABL and Fyn in K562 cells were detected by real - time fluorescence quantitative PCR . The relative changes of both mRNA expression were measured using 2 -ΔΔCt formula. RESULTS: PMA significantly inhibits the mRNA levels of BCR/ABL and Fyn in a dose - dependent manner, and the correlation of these inhibitory effects were significant. Compared with gene Molt - 4 cells, the inhibition by PMA was specific for K562 cells. The K562 cells were induced to differentiate to be pseudopodium -like ceUs. CONCLUSION: The PMA downregulates the mRNA level of Fyn by inhibiting BCR/ABL fusion gene.
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