AIM: To investigate the effect of non - alcoholic fatty liver disease ( NAFLD ) on metabolic syndrome ( MS ) - related type 2 diabetes.METHODS: Sixty - four rats were randomly divided into 8 groups: normal control groups of 3 months ( 3NC ), 6 months ( 6NC ), 9 months ( 9NC ) and 12 months ( 12NC ), and high - sucrose and high -fat groups of 3 months ( 3H ), 6 months ( 6H ), 9 months ( 9H ) and 12 months ( 12H ).The serum levels of alanine transaminase ( ALT ), free fatty acid ( FFA ), endotoxin ( LPS ), tumor necrosis factor alpha ( TNFα ), C - reactive protein ( CRP ), monocyte chemotactic protein - 1 ( MCP - 1 ), fasting plasma glucose ( FPG ) and fasting plasma insulin ( FINS ) were measured.The insulin resistance index ( HOMA - IR ) and beta cell function index( HOMA - β ) were also calculated.The specimens of liver and pancreas were prepared for microscopic observation with HE and Sudan Ⅳ staining.The visceral adipose specimens were also prepared with HE staining.The apoptosis of islet cells was detected by TUNEL.RESULTS: Compared with normal control groups, the levels of ALT, FFA, LPS, TNFα, CRP, MCP - 1, FPG, FINS and HOMA - IR in high - sucrose and high - fat groups of every phase were higher, but the level of HOMA - β in 6H group showed a compensatory increase first and then progressively decreased.Compared with normal control groups, TUNEL results showed that the number of apoptotic islet cells in high - sucrose and high - fat groups gradually increased from the 3rd to the 12th month.CONCLUSION: The NAFLD plays a trigger role in the start of MS -related type 2 diabetes.%目的:探讨非酒精性脂肪性肝病(NAFLD)在代谢综合征(MS)相关Ⅱ型糖尿病中的作用.方法:将64只大鼠随机分为8组,分别是3月正常对照组(3NC)、3月高糖高脂组(3H)、6月正常对照组(6NC)、6月高糖高脂组(6H)、9月正常对照组(9NC)、9月高糖高脂组(9H)、12月正常对照组(12NC)和12月高糖高脂组(12H).测定血浆丙氨酸氨基转移酶(ALT)、游离脂肪酸(FFA)、内毒素(LPS)、肿瘤坏死因子α(TNFα)、C反应蛋白(CRP)、巨噬细胞趋化蛋白-1(MCP-1)、空腹血糖(FPG)、空腹胰岛素(FINS),并计算胰岛素抵抗指数(HOMA-IR)、β细胞功能指数(HOMA-β).取肝组织和胰腺组织切片行苏木素-伊红(HE)染色和苏丹Ⅳ染色;内脏脂肪组织行苏木素-伊红(HE)染色.以TUNEL法检测胰岛内细胞凋亡水平.结果:与正常对照组相比,各阶段高糖高脂组大鼠血清ALT、FFA、LPS、TNFα、CRP、MCP-1、FPG、FINS和HOMA-IR水平都明显升高;而HOMA-β在第6个月出现代偿性增强后进行性衰退.高糖高脂组第3-12月胰岛凋亡细胞数量逐渐增加.结论:NAFLD(特别是脂肪肝与脂肪性肝炎)可以诱发MS相关的2型糖尿病.
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