目的:观察八肽胆囊收缩素(CCK-8)对脂多糖(LPS)诱导树突状细胞(DCs)成熟的影响.方法:采用粒-巨噬细胞集落刺激因子(GM-CSF)诱导法培养小鼠骨髓来源DCs,在LPS诱导其成熟过程中用不同浓度的CCK-8进行干预,采用流式细胞分析技术检测DCs表面主要组织相容性复合物II(MHC II)、分化群80(CD80)和分化群86(CD86)的表达;ELISA法检测DCs培养上清中白细胞介素1β(IL-1β)、白细胞介素6(IL-6)和肿瘤坏死因子α(TNF-α)的含量;MTT法检测CCK-8处理DCs对同种异体T细胞增殖反应的影响.结果:CCK-8剂量依赖性地抑制LPS诱导DCs表面CD80、CD86和MHC II表达(P<0.01,P<0.05 );CCK-8抑制LPS诱导DCs分泌IL-1β、IL-6和TNF-α(P<0.01);并且,CCK-8降低LPS诱导DCs刺激同种异体T淋巴细胞增殖的活性(P<0.05,P<0.01).结论:CCK-8对LPS诱导DCs成熟过程中的细胞表型、细胞因子分泌和抗原提呈功能有抑制作用,提示CCK-8有可能在抗感染和抵抗自身免疫性疾病过程中发挥重要调节作用.%To investigate the effect of cholecystokinin octapeptide ( CCK -8) on the maturation of dendritic cells ( DCs) stimulated by lipopolysaccharide ( LPS ). METHODS: Mouse bone marrow - derived DCs were induced by granulocyte -macrophage colony - stimulating factor (GM - CSF). When stimulated with LPS, DCs were exposed to different doses of CCK -8. The expression levels of major histocompatibility complex II ( MHC II) , cluster of differentiation 80 ( CD80) and cluster of differentiation 86 ( CD86) on DCs were determined by flow cytometry. The levels of interleukin-lp (IL - 1 (J), interleukin - 6 (IL - 6) and tumour necrosis factor - a ( TNF - a) in the supematants were detected by ELJSA. The effect of CCK -8 -treated DCs on the proliferation of allogenic T cells was analyzed by MTT method. RESULTS; CCK -8 inhibited the expression of MHC II, CD80 and CD86 in IPS - stimulated DCs in a dose - dependent manner. CCK -8 decreased the production of IL - lp, IL -6 and TNF -a from DCs stimulated by LPS. Moreover, CCK -8 impaired the effect of LPS - stimulated DCs on the proliferation of allogenic T cells. CONCLUSION; CCK -8 effectively inhibits the phenotypic and functional maturation of DCs stimulated by LPS, suggesting that CCK - 8 may play an important role in anti - infection and anti - autoimmune diseases.
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