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首页> 中文期刊> 《中国病理生理杂志》 >低氧性肾间质纤维化和游泳运动对大鼠尾加压素Ⅱ及其受体表达的影响

低氧性肾间质纤维化和游泳运动对大鼠尾加压素Ⅱ及其受体表达的影响

         
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摘要

目的:探讨慢性低氧性肾纤维化大鼠尾加压素Ⅱ(UⅡ)及其受体(GPR14)的变化,以及游泳运动在其中的作用.方法:45只雄性SD大鼠随机分成3组,即对照组、低氧组(低氧7周)和游泳组(低氧+游泳锻炼7周组,即低氧3周后,每天1 h无负重游泳4周).测定血清肌酐(Scr)、血尿素氮(BUN)和血桨UⅡ含量,以及肾组织羟脯氨酸(Hyp)含量、UⅡ及GPR14 mRNA表达和UⅡ蛋白表达.结果:(1)Scr和BUN含量:低氧组较对照组分别低18.5%和14.1%(均P< 0.05),而游泳组与低氧组间无显著差别;(2)肾组织Hyp含量:低氧组较对照组高42.9%(P<0.01),而游泳组较低氧组低26.1%(均P<0.05);(3)血浆UⅡ含量:低氧组较对照组高380.8%(P<0.01),而游泳组较低氧组低42.6%(P<0.01);(4)肾组织UⅡ mRNA表达:低氧组较对照组上调104.5%,游泳组较低氧组下调33.2%(均P<0.01);GPR14 mRNA表达:低氧组较对照组上调35.4%(P<0.01),而游泳组与低氧组间无显著差异;(5)肾组织UII蛋白的表达:低氧组明显高于对照组(P<0.01),而游泳组低于低氧组(P<0.01);(6)van Gieson染色显示低氧组肾血管胶原纤维增生明显,而游泳组明显改善.结论:慢性低氧性肾纤维化大鼠尾加压素Ⅱ及其受体的表达上调;适度游泳运动有减轻慢性低氧肾间质纤维化的作用,并下调尾加压素Ⅱ基因与蛋白的表达.%AIM: To observe the expression of urotensin II (UII) and its receptor GPR14 in rats with hypoxic tubulointerstitial fibrosis, and to explore the changes after swimming exercise. METHODS: The animal model of hypoxic renal interstitial fibrosis was established by exposing the rats to isobaric hypoxic chamber for 7 weeks (8 h/d, 7 d/week). Forty - five male SD rats were randomly divided into normal control group ( control), hypoxic 7 - week group (hypoxia) and hypoxic 3 - week and swimming without loads 4 - week group ( swimming). Serum creatinine ( Scr) and blood urea nitrogen (BUN) were measured by chemical colorimetry, and UII was detected by ELJSA. The content of hydroxyproline (Hyp) in the renal homogenate was assayed. The mRNA expression of UII and GPR14 were detected by RT - PCR. The protein level of UII was determined by the method of immunohistochemistry. Meanwhile, the renal specimens were prepared to observe renal interstitial fibrosis by van Gieson ( VG) staining. RESULTS: The content of Scr and BUN in hypoxia group was lower than that in control group by 18. 5% and 14. 1% .respectively, while there was no significant difference between swimming group and hypoxia group. The content of Hyp in hypoxia group was 42. 9% higher than that in control group, while swimming group was 26. 1% lower than that in hypoxia group. The plasma content of UII in hypoxia group was 380. 8% higher than that in control group, while swimming group was 42.6% lower than that in hypoxia group. The mRNA expression of UII in the kidneys was obviously up - regulated by 104. 5% in hypoxia group compared with control group, while it was markedly down - regulated by 33. 2% in swimming group compared with hypoxia group. The mRNA expression of GPR14 in the kidneys was significantly up - regulated by 35. 4% in hypoxia group compared with control group. However, no significant difference between hypoxia group and swimming group was observed. The UII protein level in the kidneys of hypoxia rats was distinctly higher than that in control group and lower in swimming group than that in hypoxia group. VG staining revealed that the renal interstitial fibrosis was found in hypoxia group, which was significantly alleviated by swimming exercise. CONCLUSION: The levels of UII and GPR14 increase in the kidneys of the rats with hypoxic tubulointerstitial fibrosis. Moderate swimming exercise alleviates the tubulointerstitial fibrosis induced by hypoxia and decreases the expression of UII.

著录项

  • 来源
    《中国病理生理杂志》 |2011年第11期|2189-2193|共5页
  • 作者

    毛孙忠; 邓蔚; 许芳芳; 薛峰; 胡良冈; 申屠杨萍; 范小芳; 龚永生;

  • 作者单位

    温州医学院,低氧医学研究所,肺心病研究室,浙江温州,325035;

    温州医学院,低氧医学研究所,肺心病研究室,浙江,温州,325035;

    温州医学院,机能实验教学中心,机能实验学教研室,浙江,温州,325035;

    温州医学院,低氧医学研究所,肺心病研究室,浙江温州,325035;

    温州医学院,低氧医学研究所,肺心病研究室,浙江,温州,325035;

    温州医学院,机能实验教学中心,机能实验学教研室,浙江,温州,325035;

    温州医学院,低氧医学研究所,肺心病研究室,浙江,温州,325035;

    温州医学院,机能实验教学中心,机能实验学教研室,浙江,温州,325035;

    温州医学院,低氧医学研究所,肺心病研究室,浙江,温州,325035;

    温州医学院,机能实验教学中心,机能实验学教研室,浙江,温州,325035;

    温州医学院,低氧医学研究所,肺心病研究室,浙江,温州,325035;

    温州医学院,机能实验教学中心,机能实验学教研室,浙江,温州,325035;

    温州医学院,低氧医学研究所,肺心病研究室,浙江,温州,325035;

    温州医学院,机能实验教学中心,机能实验学教研室,浙江,温州,325035;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 其他疾病;
  • 关键词

    尾加压素Ⅱ; 低氧; 肾间质; 游泳运动;

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