AIM: To construct recombinant human prostate secretory protein of94 amino acids(PSP94) expression vector. METHODS: The PSP94 cDNA was obtained from normal prostate tissue, and recombinant plasmid pUC19-PSP94 was constructed. The target gene was identified and sequenced. RESULTS: 1) The recombinant plasmid pUC19-PSP94 was constructed; 2) The mature PSP94 protein gene of 280bp was acquired by PCR; 3) The gene sequence of PSP94 was identified.CONCLUSION: The constructed plasmid pUC19-PSP94 could to be clone PSP94 successfully.%目的：作为构建前列腺分泌蛋白94（PSP94)分泌型表达质粒的基础。方法：从正常前列腺组织中钓取PSP94cDNA，构建重组质粒pUC19－PSP94，转化后提取目的基因并进行测序。结果：(1)构建了pUC19－PSP94的重组克隆质粒；(2)PCR扩增获得了280bp的PSP94成熟蛋白基因；(3)测定了PSP94的基因序列。结论：所构建的pUC19－PSP94重组质粒可成功地克隆PSP94蛋白基因。
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