AIM:To determine whether the vitreous cavity (VC) is capable of supporting the induction of deviant immune response to retinal soluble (S) antigen and to observe the influence of interleukin-1 (IL-1) on the immunologic properties of the VC. METHODS: Retinal S antigen was inoculated into the anterior chamber (AC) and VC in Wistar rats. Seven days after antigen inoculation, the recipient animals were immunized with S antigen and complete Freund's adjuvant. Delayed-type hypersensitivity (DTH) was then assessed by footpad challenge. To alter systemic immune conditions, IL-1 was administrated by intraperitoneal injection. RESULTS: Antigen-specific DTH did not develop in rats in which S antigen was injected into the AC and the VC. In contrast, strong DTH was elicited by S antigen injected into the AC and VC if IL-1 was administrated systemically for 7 consecutive days after the antigen challenge. CONCLUSION: The VC is capable of supporting immune deviation to soluble antigen by actively suppressing antigen-specific DTH. Systemic administration of exogenous IL-1 eliminates the capacity of the VC to support immune deviation inducing by soluble antigen injected locally.%目的:确立玻璃体腔(VC)是否具有支持针对视网膜可溶性抗原(S抗原)刺激诱导偏离式免疫反应的能力,并观察白细胞介素-1(IL-1)对玻璃体腔免疫特性的影响.方法:将视网膜S抗原接种于Wistar大鼠的眼前房和玻璃体腔.抗原接种后7 d,使用S抗原和完全福氏佐剂免疫受主动物.然后,通过足部刺激评估迟发型超敏反应(DTH).通过腹腔注射IL-1改变全身的免疫状态.结果:前房和玻璃体腔注射S抗原的动物没有发生抗原特异性DTH.与此相反,当全身给予IL-1时,注射于前房和玻璃体腔的S抗原则激发剧烈的DTH.结论:玻璃体腔具有通过抑制抗原特异性DTH,支持针对可溶性抗原的免疫偏离的诱导能力.全身使用外源性IL-1可以消除玻璃体腔针对局部接种可溶性抗原诱导免疫偏离的能力.
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