目的 探讨细胞外基质金属蛋白酶诱导因子（extracellular matrix metalloproteinase inducer,EMMPRIN）、基质金属蛋白酶9（matrix metalloproteinases-9,MMP-9）和P38丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)在子宫内膜异位症（endometriosis,EMs）中的表达及相关性.方法 收集2008年5-12月重庆医科大学附属第一医院收治的EMs患者44例（异位内膜44例,在位内膜38例）,对照组为同期非EMs患者在位内膜34例.应用免疫组化SABC三步法检测各组织中EMMPRIN、MMP-9、P38及磷酸化P385( p-P38)蛋白的表达,并对各蛋白表达水平进行相关性分析.结果 EMMPRIN、MMP-9、P38、p-P38蛋白在EMs异位内膜表达均高于EMs在位内膜组及对照组在位内膜,差异有统计学意义(P＜0.05)；Spearman相关分析显示,在EMs异位内膜中各蛋白表达呈正相关(P＜0.05).在EMs异位内膜组中各蛋白表达Ⅲ～Ⅳ期高于Ⅰ～Ⅱ期,差异均有统计学意义(P＜0.05).EMs在位内膜组与对照组相比,各蛋白表达差异无统计学意义(P＞0.05).结论 EMMPRIN、MMP-9在EMs异位内膜中呈显著高表达并呈正相关性,在EMs的发生发展过程中可能起重要作用.EMMPRIN诱导刺激MMP-9高表达可能与p38 MAPK途径有关.%Objective To investigate the expression and association of the extracellular matrix metalloproteinase inducer (EMMPRIN) ,the matrix metalloproteinases-9( MMP-9) ,and the P38 mitogen-activated protein kinase( MAPK) in patients diagnosed with endometriosis (EMs ). Methods The expression of EMMPRIN, MMP-9, P38, p-P38 was detected by SABC immunohistochemistry in 44 patients with EMs (44 ectopic endometrium,38 eutopic endometrium) , and compared with 34 normal endometrium with non-endometriosis. Correlation analysis was done. Results The positive expression rates of EMMPRIN, MMP-9, P38 and p-P38 in the ectopic endometrium with EMs were significantly higher than the eutopic endometrium group and the control group ( P <0.05). Spearman correlation analysis showed that positive correlation was found in EMMPRIN, MMP-9, P38 and p-P38 in the ectopic endometrium group(P<0.05).The expression level of EMMPRIN, MMP-9 ,P38 and p-P38 in the stages of III - IV displayed significantly higher than those in I ~ II in the ectopic endometrium group ( P <0.05). The positive expression rates were no significant difference between the eutopic endometrium group and the control group(P > 0.05 ). Conclusion The expression of EMMPRIN, MMP-9 in the ectopic endometrium with EMs was significantly higher and positive correlation,and they may play an important role in implantation and development. P38 MAPK may have a pathophysiological role in EMs.
展开▼
