目的 年龄相关性白内障(ARC)患者晶状体混浊程度及核的硬度与20s蛋白酶体α2亚基的蛋白表达水平及意义的关系.方法 于2015年3月至2016年7月在弋矶山医院眼科收集行超声乳化或小切口白内障摘除术的前囊膜120例(核性60例,皮质性60例).根据晶状体核的硬度及混浊程度进行分组,核性组分为A组:年轻核,B组:老核;皮质组分为A组:轻度混浊,B组:重度混浊.两种类型白内障中以A组为实验组,B组为对照组;并应用western-blot测定20s蛋白酶体α2亚基在两组ARC患者晶状体前囊膜中的表达水平.结果 与A组相比较,B组20s蛋白酶体α2亚基表达水平明显降低,核性(0.99±0,02) VS (0.58±0.08)差异有统计学意义(P＜0.01),皮质性(1.26±0,15) vS(0.89±0.09)差异有统计学意义(P＜0.05).ARC患者晶状体混浊程度及核的硬度与20s蛋白酶体α2亚基含量呈显著负相关.结论 ARC患者前囊膜中20s蛋白酶体α2亚基的蛋白表达含量随晶状体混浊程度及核的硬度加深而降低.%Objective To identify the relationship between the degree of lens opacity and the hardness of nuclear and the protein expression level of 20s proteasome α2 subunit in age-related cataract patients.Methods Phacoemulsification or small incision cataract extraction was performed in 120 cases (60 cases of nuclear and 60 cases of cortical) and collected these anterior capsules.According to the degree of hardness and opacity of the lens nucleus,the nucleus was divided into group A:young nucleus,group B:old nuclear;cortical was divided into group A:mild opacity,group B:severe haze.Two types of cataract in group A,group B;and Western-blot was used to determine the 20s proteasome subunit alpha 2 expression levels in two groups of patients with ARC in the anterior lens capsule;experimental research work from July 2015 to March 2016.Results Compared with group A,the group B,20s proteasome subunit a2 expression levels were significantly lower (nuclear (0.99±0.02) VS (0.58±0.08) P ＜0.01,cortical (1.26±0.15) VS (0.89±0.09),P ＜0.05,with the degree of lens opacity and nuclear hardness with the 20s proteasome α2 subunit content in age-related cataract patients was negatively correlated.Conclusions The protein expression levels of 20s protein α2 subunit in the anterior capsule of age-related cataract patients decrease with the degree of lens opacity and the hardness of the nucleus.
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