Non-structural protein 2 (Nsp2) is the most variable region in porcine reproductive and respiratory syndrome virus (PRRSV) and it has multiple roles in polypeptide processing and replication complex formation. To investigate the role of Nsp2 in the viability and replication of PRRSV in vitro, three PRRSV cDNA clones of different deletion in the region of Nsp2 were constructed by reverse genetic manipulation, including the cDNA clones with encoding sequence for the deletion of 210 consecutive amino acids (pOK-AaBCD), 105 amino acids (pOK-AbBCD) and 96 amino acids (pOK-AcBCD) in the Nsp2 gene of PRRSV (XH-GD strain), by respectively. However, only the recombinant virus with deletion of 105 amino acids (XH-GD-A105) was rescued in MARC-145 cells transfected with pOK-AaBCD, but not other two cDNA clones. The result would be useful for further study on the relationship between Nsp2 gene deletions and pathogenic of PRRSV in pigs.%为研究Nsp2编码区不同区域缺失对病毒体外复制的影响,本研究在猪繁殖与呼吸综合征病毒(PRRSV) XH-GD株反向遗传操作平台的基础上构建了pOK-AaBCD、pOK-AbBCD、pOK-AcBCD等3种Nsp2编码区部分缺失的感染性重组质粒.将这3种感染性质粒分别转染Marc-145细胞进行不同Nsp2缺失株的病毒拯救,结果显示,只有在Nsp2编码区缺失105个氨基酸的缺失株XH-GD-A 105能够在Marc-145细胞中增殖,表明这一缺失区域为病毒复制的非必需,而其他两种感染性质粒则未拯救出相应的缺失重组病毒.本研究为进一步研究Nsp2的基因序列的缺失与PRRSV毒力之间的关系奠定了基础.
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