目的:探讨大鼠经高脂膳食复制胰岛素抵抗模型后,运动对其的改善作用及脂肪细胞分化在其中的影响.方法:SD纯系雄性大鼠40只,随机分为对照组(C)、高脂组(H)、高脂运动组(HE)和普通膳食运动组(E),各运动组进行8周跑台训练,对比各组的胰岛素敏感性指标和脂肪细胞分化各指标.结果:8周高脂膳食成功复制大鼠胰岛素抵抗模型.大鼠脂肪细胞分化各指标变化:PPARγ在E组高于C组(P＜0.01),H组高于C组；C/EBPα在H组高于其他各组(P＜0.01),E组高于C组,HE组低于H组；E组脂肪细胞分化程度最高(P＜0.01)；C组增殖指数最高,与H组和E组相比具有显著性差异(P＜ 0.0I;P＜ 0.05),HE组高于H组和E组(P＜0.05)；E组脂肪细胞体积小于C组(P＜0.01),细胞数量高于C组；H组细胞体积小于C组(P＜0.01),数量也增加(P＜0.01),HE组与H组相比脂肪细胞体积较大(P＜0.01),且数量减少.脂肪细胞分化指标与胰岛素抵抗指标间具有一定的相关关系.结论:8周跑台运动可以改善由高脂膳食喂养引起的大鼠胰岛素抵抗现象,具体机制可能与其脂肪细胞分化的变化相关.%Objective: To study the effects of aerobic exercise on insulin insistence in the rats feed by high-fiat-diet and discuss whether the effect acted via the adipocyte differentiation.rnMethod:Forty male SD rats were randomly assigned to four groups: control(C), high-fat diet control(H), high-fat diet control+exercise training(HE) and exercise training(E) group. Rats in HE and E groups were given treadmill exercise training. Insulin sensitivity and adipocyte parameters were evaluated.rnResult :The insulin resistence rat model was duplicated successfully by high-fat-diet. The changes of adipose differentiation were as follows: PPAR y protein of E group was higher than that of C group(P<0.01); that of H group was higher than C group, that of HE group was lower than H group. C/EBP α protein of H group was higher than that of other groups(P<0.01); that of E group was higher than C group, that of HE group was lower than H group. The adipose differentiation of E group was the highest compared with C and H groups(P<0.01); the proliferation index of C group was the highest compared with H and E groups(P<0.01 and P<0.05), that of HE group was lower than H and E groups(P<0.05). In E group size of adipose cells was smaller than that of C group(P<0.01), number of cells were more than that of C group; in H group size of adipose cells was smallerrnthan that of C group (P<0.01), and the number increased(P<0.0l); in HE group size of adipose cells was largerrnthan that of G group(P<0.01) and the number decreased. There was correlation between adipocyte differentiationrnand insulin resistance.rnConclusioniTreadmill exercise for 8 weeks could improve the insulin resistence induced by high-fat diet and therninner mechanism may be related to adipose differentiation.
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