背景:前期研究观察到神经干/祖细胞表达甲酰基肽受体(formyl peptide receptors,FPRs),并证实FPRs能促进神经干/祖细胞迁移和诱导神经干细胞向类神经元分化.损伤的组织中存在FPRs配体,然而不同的配体与FPRs的结合可能导致不同、甚至相反的生物学效应.目的:探讨脊髓损伤产生的配体与FPRs作用后对神经干/祖细胞向类神经元分化的影响.方法:免疫荧光染色、Western blotting和流式细胞仪分析FPRs在神经干/祖细胞中的表达;免疫荧光染色共聚焦显微镜观察脊髓匀浆对FPR1或FPR2阳性神经干/祖细胞分化的影响.结果与结论:①部分神经干/祖细胞表达FPR1和FPR2,不仅在细胞膜上有表达,在细胞质中也有表达,FPR1的表达水平明显低于FPR2的表达水平;②脊髓匀浆液能够使FPR1或FPR2阳性神经干/祖细胞分化后产生的β- tubulinⅢ 阳性神经元比例升高、GFAP阳性星形胶质细胞比例降低,这种作用能够被FPR1或FPR2阻断剂Boc2或WRW4阻断;③这些实验结果说明,脊髓匀浆液能够促进FPR1或FPR2阳性神经干/祖细胞向类神经元分化,抑制其向星形胶质细胞分化,同时这种作用具有特异性.%BACKGROUND: Previous studies have observed the expression of formyl peptide receptors (FPRs) in neural stem/progenitor cells (NSPCs) and confirmed that FPRs can promote the migration of NSPCs and induce them to differentiate into neurons. FPRs ligands are present in damaged tissues, but the binding of different ligands with FPRs may lead to different and even opposite biological effects. OBJECTIVE: To investigate the effect on the differentiation of NSPCs into neurons after the binding of the ligands produced following spinal cord injury with FPRs. METHODS: Immunofluorescence staining, western blot and flow cytometry were used to analyze the expression of FPRs in NSPCs. Immunofluorescent staining with confocal microscope detection was used to analyze the effect of homogenates of the spinal cord on the differentiation of FPR1+or FPR2+NSPCs into neurons. RESULTS AND CONCLUSION: Some of NSPCs expressed FPR1 and FPR2, not only on the cell membrane, but also in the cytoplasm. The expression level of FPR1 was obviously lower than that of FPR2. The homogenate group for FPR1+or FPR2+NSPCs could produce more β-III tubulin-positive cells and fewer GFAP-positive astrocytes, and the effects could be blocked by FPR1 or FPR2 inhibitor Boc2 or WRW4. These experimental findings show that the spinal cord homogenate can induce FPR1 or FPR2 positive NSPCs to differentiate into neurons and inhibit their differentiation to astrocytes, and moreover, this effect is specific.
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