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首页> 中文期刊> 《中国组织工程研究》 >正常和类风湿关节炎关节成纤维样滑膜细胞迁移、侵袭能力的差异

正常和类风湿关节炎关节成纤维样滑膜细胞迁移、侵袭能力的差异

         
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背景:在类风湿关节炎的发病过程中,其滑膜衬里层的成纤维样滑膜细胞的病理生理学行为涉及细胞的增殖、侵袭、迁移、凋亡和破骨作用.目的:比较类风湿关节炎关节成纤维样滑膜细胞(MH7A)与正常关节成纤维样滑膜细胞(HFLS)迁移和侵袭能力.方法:利用Transwell细胞迁移和侵袭实验比较2种细胞的迁移、侵袭能力,设计11种miRNA序列特异性引物,按SYBR?PrimeScript?miRNA RT-PCR Kit说明书进行实时定量聚合酶链式反应检测类风湿关节炎相关miRNAs的表达.结果与结论:①Transwell细胞迁移、侵袭实验显示MH7A迁移和袭能力较HFLS明显增强;与HFLS相比,MH7A细胞中miR-132、-155、-203、-223、-124的表达上调;而miR-15a、-16、18a、-19a、-26a、-146a的表达下调.②结果说明,MH7A迁移和侵袭能力较HFLS明显增强;与HFLS相比,MH7A中类风湿关节炎相关的11种miRNAs表达水平的改变,可能通过相应的机制调节类风湿关节炎成纤维样滑膜细胞的迁移和侵袭,参与类风湿关节炎的发病.%BACKGROUND:Fibroblast-like synoviocytes (FLS) in the synovial lining layer are related to the cell proliferation, invasion, migration and apoptosis as well as bone resorption in rheumatoid arthritis. OBJECTIVE:To compare the migration and invasion abilities of FLS (MH7A) in rheumatoid arthritis and normal FLS (HFLS). METHODS:The capacities of cell migration and invasion were evaluated by Transwell cell migration and invasion assays. The primers of the indicated microRNAs were designed and synthesized, and the expression levels of miRNAs were determined by real-time PCR according to the SYBR?PrimeScript?miRNA RT-PCR Kit instruction. RESULTS AND CONCLUSION:MH7A possessed stronger migration and invasion abilities than HFLS. Compared with HFLS, obviously upregulated miR-132, -155, -203, -223 and -124, and significantly downregulated miR-15a, -16, 18a, -19a, -26a and -146a were found in MH7A. These findings suggest that the differentially expressed 11 kinds of rheumatoid arthritis-associated miRNAs participate in the pathogenesis of rheumatoid arthritis probably by enhancing the migration and invasion capacities of MH7A.

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