BACKGROUND: Traditional dialysis membranes are mostly synthesized by organic polymer materials. Although these materials can meet the needs of treatment, the pore size range is large and the microtubules are not uniform, which can cause protein clogging on the dialysis membrane. Therefore, it is difficult to achieve the expected therapeutic effect.OBJECTIVE: To study the hemocompatibility of titanium dioxide nanotube bio-dialysis membrane.METHODS: High-strength titanium dioxide nanotube films were prepared by anodic oxidation method, the bottom of the nanotubes was etched by HF gas, and the LLC-PK1 and ECV304 cells were seeded onto the prepared titanium dioxide nanotube array membranes to construct two kinds of titanium dioxide nanotube bio-dialysis membranes. Hemolysis test,dynamic clotting time test and platelet adhesion test were used to determine the hemocompatibility of the prepared titanium dioxide nanotube bio-dialysis membrane.RESULTS AND CONCLUSION: In the hemolysis test, the hemolysis rates of the titanium dioxide nanotube array membranes carrying LLC-PK1 and ECV304 cells were lower than that of the polyurethane material (0.30％, 0.34％,0.56％; P < 0.05). In the dynamic clotting test (20, 40, 70 minutes), the dynamic clotting time of the titanium dioxide nanotube array membranes carrying LLC-PK1 and ECV304 cells were significantly longer compared with the polyurethane material (P < 0.05). In the platelet adhesion test, the platelet adhesion rate showed no significant difference between the polyurethane material and the titanium dioxide nanotube array membranes carrying LLC-PK1 and ECV304 cells (P > 0.05). These findings indicate that the titanium dioxide nanotube bio-dialysis membrane possesses good hemocompatibility.%背景:传统的透析膜多由有机聚合物材料合成,这些材料虽然能满足治疗需要,但材料孔径尺寸范围较大,微管不均匀,容易造成蛋白质在透析膜上淤塞,难以达到预期的治疗效果.目的:分析二氧化钛纳米管生物透析膜的血液相容性.方法:根据阳极氧化法制备高强度的二氧化钛纳米管薄膜,利用HF气体对纳米管底部进行腐蚀,获得两端通透的二氧化钛纳米管阵列薄膜,将LLC-PK1细胞和ECV304细胞分别接种于二氧化钛纳米管阵列薄膜上,获得两种二氧化钛纳米管生物透析膜.①溶血实验:将两种二氧化钛纳米管生物透析膜及聚氨酯材料分别浸泡于生理盐水与蒸馏水中,检测溶血率;②动态凝血时间实验:将涂过兔血的两种二氧化钛纳米管生物透析膜及聚氨酯材料分别与氯化钙混合,计算动态凝血时间;③血小板黏附实验:将富血小板血浆分别滴于两种二氧化钛纳米管生物透析膜及聚氨酯材料上,计算血小板黏附率.结果与结论:①溶血实验:接种LLC-PK1细胞、ECV304细胞通透二氧化钛纳米管薄膜的溶血率均低于聚氨酯材料(0.30％,0.34％,0.56％,P<0.05);②动态凝血时间实验:接种LLC-PK1细胞、ECV304细胞通透二氧化钛纳米管薄膜20,40,70 min的动态凝血时间显著长于聚氨酯材料(P<0.05);③血小板黏附实验:接种LLC-PK1细胞、ECV304细胞通透二氧化钛纳米管薄膜的与聚氨酯材料相比差异无显著性意义(P>0.05);④结果表明:二氧化钛纳米管生物透析膜具有良好的血液相容性.
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