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首页> 中文期刊> 《中国组织工程研究》 >自组装纳米纤维凝胶培养基中神经干细胞的增殖与分化*★

自组装纳米纤维凝胶培养基中神经干细胞的增殖与分化*★

         
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BACKGROUND: Isoleucine-lysine-valine-alanine-valine has good biological activity, and under certain conditions, it can self-assemble into isoleucine-lysine-valine-alanine-valine nanofiber hydrogel scaffold which is a natural biomimetic material for the spinal cord matrix. OBJECTIVE: To observe the effects of self-assembled isoleucine-lysine-valine-alanine-valine nanofiber gel on proliferation and differentiation of bone marrow derived neural stem cells in vitro. METHODS: Passage 2 bone marrow derived neural stem cells were co-cultured with isoleucine-lysine-valine-alanine-valine as experimental group, neural stem cells cultured alone counted as control group, and isoleucine-lysine-valine-alanine-valine was set as blank control group. Cel proliferation rate and differentiation proportion of neural stem cells into neurons were measured at 1, 3, 5, 7, 10, 14 days after culture using Cel Counting Kit-8 and flow cytometry, respectively. 3-(4,5-Dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assay was used for detecting cytotoxicity of passage 2 bone marrow derived neural stem cells from new born Sprague-Dawley rats. RESULTS AND CONCLUSION: Cel proliferation rate and differentiation proportion of neural stem cells into neurons were significantly higher in the experimental group than in the control groups at each time (P < 0.05). Cel proliferation in the experimental and control groups peaked at day 7. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay detected no cytotoxicity of the isoleucine-lysine-valine-alanine-valine. Taken together, isoleucine-lysine-valine-alanine-valine nanofiber gel has a better biological activity, and can increase the differentiation proportion of neural stem cells into neurons.%　　背景：异亮氨酸-赖氨酸-缬氨酸-丙氨酸-缬氨酸具有良好的生物活性，在特定条件下可自组装成含IKVAV 纳米纤维凝胶支架，是一种天然的脊髓基质仿生材料。目的：进一步观察体外自组装异亮氨酸-赖氨酸-缬氨酸-丙氨酸-缬氨酸纳米纤维凝胶对骨髓源性神经干细胞增殖及向神经元分化的影响。方法：取第2代新生 SD 大鼠骨髓源性神经干细胞与自组装异亮氨酸-赖氨酸-缬氨酸-丙氨酸-缬氨酸纳米纤维凝胶共培养作为实验组，设置神经干细胞单独培养为对照组、单独自组装异亮氨酸-赖氨酸-缬氨酸-丙氨酸-缬氨酸纳米纤维凝胶为空白对照组，CCK-8法及流式细胞仪检测培养1，3，5，7，10，14 d 的细胞增殖与神经干细胞向神经元分化的比例；MTT 法检测自组装异亮氨酸-赖氨酸-缬氨酸-丙氨酸-缬氨酸纳米纤维凝胶对第2代新生 SD 大鼠骨髓源性神经干细胞的毒性。结果与结论：实验组不同时间点细胞增殖率及向神经元分化的细胞比例均高于对照组(P <0.05)，两组细胞增殖均于第7天达峰值。MTT 法检测结果显示自组装异亮氨酸-赖氨酸-缬氨酸-丙氨酸-缬氨酸纳米纤维凝胶无细胞毒性。表明异亮氨酸-赖氨酸-缬氨酸-丙氨酸-缬氨酸纳米纤维凝胶有较好的生物活性，可促进神经干细胞的增殖，提高神经干细胞向神经元分化的比例。

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