BACKGROUND: Previously, sub-health and chronic fatigue model is constructed with complex physical factors, but sub-healthfatigue rat model is rarely report.OBJECTIVE: To observe the sub-health fatigue rat models constructed by sleep deprivation combined with exhaustiveweight-loading swimming exercise.METHODS: Thirty-two male rats were randomly divided into four groups by weight. Control group, 1-day model, 4-day model and5-day model group. Rats in the model group were used to establish the model of sub-health fatigue by being deprived of sleep for20 hours, and then the rats were required to conduct exhaustive swimming exercise with loading 5% of rat body weight. Theexhaustive swimming time was recorded. Each group was preformed with fasting for one day in the end of the experiment, andtoke the blood biochemistry.RESULTS AND CONCLUSION: Compared the last exhaustive swimming time with the first, the exhaustive swimming time of4-day model and 5-day model group was shorter significantly (P < 0.05). The levels of blood Alpha-hydroxybutyratedehydrogenase and blood urea nitrogen in 5-day group was increased compared with the rest groups (P < 0.05). The levels ofblood cholesterol aspartate aminotransferase, alanine aminotransferase, creatinine and triglyceride were decreased (P < 0.05).Exhaustive swimming exercise with loading 5% of rat body weight and sleep deprivation for 20 hours might establish a rat modelof sub-health fatigue.%背景:有研究用复合因素构建了亚健康及慢性疲劳大鼠模型,但疲劳型亚健康大鼠模型的构建较罕见.目的:采用力竭游泳复合睡眠剥夺构建疲劳型亚健康大鼠模型,探讨最佳的疲劳型亚健康大鼠模型制备方法.方法:将大鼠负重5%力竭游泳并睡眠剥夺20 h 建立疲劳型亚健康模型,记录力竭游泳时间,各组实验结束禁食1 d,行血液生化检查.结果与结论:建模后4,5 d 大鼠末次力竭游泳时间显著减少(P < 0.05).建模后5 d,大鼠血α-羟丁酸脱氢酶、尿素氮、血肌酸激酶升高(P < 0.05)、总胆固醇降低,门冬氨酸氨基转移酶、丙氨酸氨基转移酶、血清肌酐、三酰甘油水平均降低(P < 0.05).结果证实,负重5%的力竭游泳复合20 h 睡眠剥夺持续4 d 即可成功制备疲劳型亚健康大鼠模型.
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