Objective: To observe the effects of tonifying qi and detoxication prescription( niuhuangshen ) contained serum on rats' liver stem cell line WB - F344 proliferation, differentiation and apoptosis, explore the mechanism.rnMethods: The effects of different concentrations of niuhuangshen contained serum on proliferation of WB - F344 were determined by MTT colorimetry; selecting the optimal concentration of niuhuangshen contained serum on proliferation, the effects of niuhuangshen contained serum on the expressions of HGFR, EGF, EGFR, TGFβ1,R and TGFβ2R in WB - F344 cells were detected by Western blotting, WB - F344 cells apoptosis was determined by flow cytometer .rnResults: MTT results showed 10% and 20% concentration of niuhuangshen contained serum could promote proliferation of WB -F344, the optimal concentration for proliferation was 10% ( P <0. 01 ); Western blotting results showed that niuhuangshen contained serum could up - regulate the expression HGFR, EGF, EGFR, TGFβ1 R and TGFβ2 R in WB - F344 cells( P < 0. 01 ); and apoptosis rate of WB - F344 cell significantly decreased after intervened by drug - containing serum ( P <0. 01 ).rnConclusion: The prescription of tonifying qi and detoxication( niuhuangshen ) contained serum can promote rats' liver stem cell proliferation , differentiation, and inhibiting its apoptosis to stimulate regeneration of liver cell by acting on cytokine system.%目的:研究牛黄参含药血清对大鼠肝干细胞系WB-F344增殖、分化及凋亡的影响,探讨其作用机制.方法:MTT法检测不同浓度含药血清对WB-F344 增殖的影响;取增殖的最佳浓度,Western blot检测含药血清对WB-F344细胞因子HGFR、EGF、EGFR、TGFβ1R、TGFβ2R表达的影响;流式细胞仪检测含药血清对WB-F344细胞凋亡的影响.结果:MTT结果显示10%、20%浓度的含药血清均能促进WB-F344细胞的增殖,且以10%为增殖的最佳浓度(P＜0.01);Western blot结果显示经10%的含药血清干预后,WB-F344细胞HGFR、EGF、EGFR、TGF-β1R、TGF-β2R的表达量上调(P＜0.01);流式细胞仪检测结果则显示含药血清干预后WB-F344细胞凋亡率显著下降(P＜0.01).结论:牛黄参含药血清可促进大鼠肝干细胞的增殖、分化,并抑制其凋亡,这可能是其抗肝损伤、促进肝再生的作用机理之一.
展开▼
