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首页> 中文期刊> 《中华创伤杂志（英文版）》 >Effect of glycyrrhizin on traumatic brain injury in rats and its mechanism

Effect of glycyrrhizin on traumatic brain injury in rats and its mechanism

         
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摘要

Objective:To investigate the neuroprotective effects of glycyrrhizin (Gly) as well as its effect on expression of high-mobility group box 1 (HMGB 1) in rats after traumatic brain injury (TBI).Methods:Male Sprague-Dawley rats were randomly divided into three groups:sham group,TBI group,and TBI+Gly group (n=36 per group).Rat TBI model was made by using the modified Feeney's method.In TBI+Gly group,Gly was administered intravenously at a dosage of 10 mg/kg 30 min after TBI.At 24 h after TBI,motor function and brain water content were evaluated.Meanwhile,HMGB 1/HMGB 1 receptors including toll-like receptor 4 (TLR4) and receptor for advanced glycation end products (RAGE)uclear factor-κ B(NF-κ B) signaling pathway and inflammatory cytokines in the injured brain tissues were detected using quantitative real-time polymerase chain reaction,western blot,electrophoretic mobility shift assay and enzyme-linked immunosorbent assay.Furthermore,HMGB 1,RAGE and TLR4 immunohistochemistry and apoptosis were analyzed.Results:Beam walking performance impairment and brain edema were significantly reduced in TBI+Gly group compared with TBI group; meanwhile,the over-expressions of HMGB 1/HMGB 1 receptors (TLR4 and RAGE)/NF-κB DNA-binding activity and inflammatory cytokines were inhibited.The percentages of HMGB 1,RAGE and TLR4positive cells and apoptotic cells were respectively 58.37％±5.06％,54.15％±4.65％,65.50％± 4.83％,52.02％± 4.63％ in TBI group and 39.99％±4.99％,34.87％±5.02％,43.33％±4.54％,37.84％±5.16％ in TBI+Gly group (all P＜0.01 compared with TBI group).Conclusion:Gly can reduce secondary brain injury and improve outcomes in rat following TBI by down-regulation of HMGB 1/HMGB 1 receptors (TLR4 and RAGE)/NF-κ B-mediated inflammatory responses in the injured rat brain.

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  • 来源
    《中华创伤杂志（英文版）》 |2014年第1期|1-7|共7页
  • 作者

    Gu Xiangjin; Xu Jin; Ma Banyou; Chen Gong; Gu Peiyuan; Wei Dong; Hu Weixing;

  • 作者单位

    Department of Neurosurgery, Jiangning Hospital Affiliated to Nanjing Medical University, Nanjing 211100, China;

    Department of Neurosurgery, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China;

    Department of Neurosurgery, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China;

    Department of Neurosurgery, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China;

    Department of Neurosurgery, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China;

    Department of Neurosurgery, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China;

    Department of Neurosurgery, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China;

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