Anti-inflammatory effect of erythropoietin pretreatment on cardiomyocytes with hypoxia/reoxygenation injury and the possible mechanism

QIN Chuan; XIAO Ying-bin; ZHONG Qian-jin; CHEN Lin; WANG Xue-feng

首页> 中文期刊> 《中华创伤杂志（英文版）》 >Anti-inflammatory effect of erythropoietin pretreatment on cardiomyocytes with hypoxia/reoxygenation injury and the possible mechanism

Anti-inflammatory effect of erythropoietin pretreatment on cardiomyocytes with hypoxia/reoxygenation injury and the possible mechanism

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Objective: To investigate the anti-inflammatory effect of erythropoietin (EPO) pretreatment on cardiomyocytes ex-posed to hypoxia/reoxygenation injury (H/R) and explore the possible mechanism. Methods: The cultured neonatal rats' ventricular cardiomyocytes were divided randomly into 4 groups, con-trol group (C group), EPO pretreatment group (E group), EPO and pyrrolidine dithiocarbamate (PDTC) pretreatment group (EP group) and PDTC pretreatment group (P group). After 24 hours' pretreatment, the cardiomyocytes were exposed to H/R. After pretreatment and H/R, the expression of tumor necrosis factor- α (TNF- α ) gene in all the groups was detected by RT-PCR and Western blot. The nuclear factor- KB (NF- kB) activity was detected by electrophoretic mobility shift assay (EMSA) and the inhibitor-kBα (I- kBα)protein level was detected by Western blot. Results: The decrement of I- K B α protein and the in-creasing NF- kB activity were found in cardiomyocytes pre-treated with EPO before H/R compared to other groups (t=-3.321,4.183, P<0.01). However, after H/R, NF- kB activity and ex-pression of TNF- α gene were significantly reduced, I- k B α protein expression was increased in cardiomyocytes of E group compared to other groups (t=-3.425, 3.687, 3.454, P<0.01). All theses changes caused by EPO pretreatment were eliminated by the intervention of PDTC (an antagonist to NF- kB) dur-ing pretreatment. Conclusions: EPO pretreatment can inhibit the activa-tion of NF- kB and upregulation of TNF- α gene in cardiomyocytes exposed to H/R through a negative feed-back of NF- k B signaling pathway, and thus produces the anti-inflammatory effect. This might be one of the ways EPO produces the anti-inflammatory effect.

著录项

来源
《中华创伤杂志（英文版）》 |2008年第6期|352-358|共7页
作者
QIN Chuan; XIAO Ying-bin; ZHONG Qian-jin; CHEN Lin; WANG Xue-feng;
展开▼
作者单位

Department of Cardiovascular Surgery, Xinqiao Hospital,Third Military Medical University, Chongqing 400037, China;

Department of Cardiovascular Surgery, Xinqiao Hospital,Third Military Medical University, Chongqing 400037, China;

Department of Cardiovascular Surgery, Xinqiao Hospital,Third Military Medical University, Chongqing 400037, China;

Department of Cardiovascular Surgery, Xinqiao Hospital,Third Military Medical University, Chongqing 400037, China;

Department of Cardiovascular Surgery, Xinqiao Hospital,Third Military Medical University, Chongqing 400037, China;

展开▼
原文格式 PDF
正文语种 chi
中图分类外科学;
关键词
Erythropoietin; Myocytes, cardiac; Tumor necrosis factor-alpha;

机译：促红细胞生成素;心肌细胞;肿瘤坏死因子-α;

Anti-inflammatory effect of erythropoietin pretreatment on cardiomyocytes with hypoxia/reoxygenation injury and the possible mechanism

摘要

著录项

相关主题

期刊订阅