Effects of Ginkgo biloba extract on lipid peroxidation and apoptosis after spinal cord ischemia/reperfusion in rabbits

樊立宏; 王坤正; 程斌

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Effects of Ginkgo biloba extract on lipid peroxidation and apoptosis after spinal cord ischemia/reperfusion in rabbits

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＜正＞Objective: To study the effects of Ginkgo biloba extract （ GBE） on lipid peroxidation and apoptosis after spinal cord ischemia/reperfusion （I/R） in rabbits. Methods: Spinal cord I/R injury model was established according to the description of Erten et al. A total of 27 New Zealand white rabbits were divided into three groups randomly: a sham group （9 rabbits treated with sham operation but without aortic occlusion） . a model group （9 rabbits treated with aortic occlusion and volume-matched saline） , and a GBE group （9 rabbits treated with aortic occlusion and Ginaton （100 mg/ kg） injected 30 minutes before aortic clamping and at the onset of reperfusion）. The neurological outcomes were evaluated at 24 and 48 hours after reperfusion, respectively. The spinal cord malondialdehyde （ MDA） level, superoxide dismutase （ SOD） were then detected. Neural cell apoptosis was determined by terminal deoxynucleotidyl transferase （ TdT ） -mediated dUTP-fluorescence nick end labeling （ TUNEL） method and the expression of bcl-2 and bax were examined histologically in the spinal cord with immunohistochemistry. Results: I/R produced a significant decrease in neurological scoring. The motor scores of the GBE group were significantly higher than those of the model group at 24 and 48 hours after reperfusion （P＜0.05）. Compared with the model group, GBE ameliorated the down-regulation of SOD and produced a significant reduction of the MDA level （ P ＜0.01）. The positive cells for TUNEL in the model group were much more than those of the GBE group （P＜0.01）. The bcl-2 was up-regulated after I/R, especially in the GBE group （P＜0.01）. The up-regulation of bax was greatly diminished by GBE （P＜0.01）. Conclusions: GBE has protective effects against spinal cord I/R injury, and the mechanism may be that it can scavenge oxygen free radicals and inhibit the apoptosis of neural cells.

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《中华创伤杂志：英文版》 |2006年第2期|77-81|共5页
作者
樊立宏; 王坤正; 程斌;
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作者单位

Department of Orthopedics Second Affiliated Hospital Xi’an Jiaotong University;

Xi’an 710004;

China;

Department of Orthopedics Second Affiliated Hospital;

Xi’an Jiaotong University;

Xi’an 710004;

China;

Department of Orthopedics Second Affiliated Hospital;

Xi’an;

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原文格式 PDF
正文语种 chi
中图分类中药实验药理;
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机译：缺血;再灌注;凋亡;脊髓;脂质过氧化;银杏叶提取物;

Effects of Ginkgo biloba extract on lipid peroxidation and apoptosis after spinal cord ischemia/reperfusion in rabbits

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