Bcl-2 gene therapy for apoptosis following traumatic brain injury

杨小锋; 郑学胜; 刘伟国; 冯军峰

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Bcl-2 gene therapy for apoptosis following traumatic brain injury

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＜正＞Objective: Toinvestigatethetherapeuticeffect of Bcl-2 fusion protein on apoptosis in brain following traumatic brain injury. Methods: Bcl-2 gene was cloned by RT-PCR. Bcl-2 and EGFP genes were linked together and inserted into pAdeno-X vector. This recombinant vector was packaged into infectious adenovirus in HEK293 cells. Ninety Wistar rats were assigned randomly into experimental group （n=45） and control group （n=45）. All rats were subjected to traumatic brain injury. Then recombinant adenovirus （for experimental group） or saline （for control group） was injected into the traumatic brain. The expression of Bcl-2 fusion protein was investigated by Western blotting, immunohistochemistry and fluorescence microscopy. Apoptosis in the injured brain was studied by TUNEL. Animals’ behavior capacity was evaluated by tiltboard test. Results: In the experimental group, many fluorescent cells were found around the traumatic locus, which were also proven to be Bcl-2-positive by immunohistochemistry. On the contrary, few Bcl-2-positive cells and no fluorescent cell were detected in the control group. Bcl-2 expression of experimental group was much higher than that of control group, which was illustrated by Western blotting. The apoptosis index of experimental group was 0. 027±0. 005, and that of control group was 0.141±0.025 （P ＜0.01）. Two weeks after injury, animals of the experimental group behaved better than those of the control group. Conclusions: A recombinant adenovirus vector expressing Bcl-2 fusion protein has been constructed. Bcl-2 fusion protein can suppress apoptosis and promote cell survival. Moreover, the behavior recovery of the injured animal is promoted. Bcl-2 fusion protein provides a way to track the target cells in vivo.

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《中华创伤杂志：英文版》 |2006年第5期|276-281|共6页
作者
杨小锋; 郑学胜; 刘伟国; 冯军峰;
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作者单位

Department of Neurosurgery Second Affiliated Hospital;

College of Medicine;

Zhejiang University;

Hangzhou 310009;

China;

Department of Neurosurgery;

Second Affiliated Hospital;

College of Medicine;

Zhejiang University;

Hangzhou 310009;

China;

Department of Neurosurg;

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原文格式 PDF
正文语种 chi
中图分类颅脑;
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机译：脑损伤;凋亡;Bcl-2融合蛋白;

Bcl-2 gene therapy for apoptosis following traumatic brain injury

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