Adipose tissues differentiated by adipose-derived stemcells harvested from transgenic mice

LU Feng; GAO Jian-hua; Rei Ogawa; Hiroshi Mizuro; Hiki Hykusoku

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Adipose tissues differentiated by adipose-derived stemcells harvested from transgenic mice

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Objective: To induce adipocyte differentiation in vitro by adipose-derived stromal cells (ASCs) harvested from transgenic mice with green fluorescent protein (GFP)and assess the possibility of constructing adipose tissues via attachment of ASCs to type Ⅰ collagen scaffolds.Methods: Inguinal fat pads from GFP transgenic mice were digested by enzymes for isolation of ASCs (primary culture). After expansion to three passages of ASCs, the cells were incubated in an adipogenic medium for two weeks, and the adipocyte differentiation by ASCs in vitro was assessed by morphological observation and Oil Red O staining. Then they were attached to collagen scaffolds and co-cultured for 12 hours, followed by hypodermic implantation to the dorsal skin of nude mice for 2 months. The newly-formed tissues were detected by HE staining.Results: The cultured primary stem cells were fibroblast-like and showed active proliferation. After being incubated in an adipocyte differentiation medium, the lipid droplets in the cytoplasm accumulated gradually and finally developed into mature adipocytes, which showed positive in Oil Red O staining. A 0.5-cm3 new tissue clot was found under the dorsal skin of the nude mice and it was confirmed as mature adipose tissues by fluorescent observation and HE staining.Conclusions: ASCs can successfully differentiate adipose tissues into mature adipocytes, which exhibit an adipocyte-like morphology and express as intracytoplasmic lipid droplets. It is an efficient model of adipose tissues engineered with ASCs and type Ⅰ collagen scaffolds.

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《中华创伤杂志（英文版）》 |2006年第6期|359-364|共6页
作者
LU Feng; GAO Jian-hua; Rei Ogawa; Hiroshi Mizuro; Hiki Hykusoku;
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作者单位

Department of Plastic and Reconstructive Surgery, Nanfang Medical University, Guangzhou 510515, China;

Department of Plastic and Reconstructive Surgery, Nanfang Medical University, Guangzhou 510515, China;

Departnent of Plastic and Reconstructive Surgery, Nippon Medical School, Tokyo, Japan;

Departnent of Plastic and Reconstructive Surgery, Nippon Medical School, Tokyo, Japan;

Departnent of Plastic and Reconstructive Surgery, Nippon Medical School, Tokyo, Japan;

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原文格式 PDF
正文语种 chi
中图分类外科学;
关键词
Adipose tissues; Stem cell transplantation; Mice, transgenic;

机译：脂肪组织;干细胞移植;小鼠;转基因;

Adipose tissues differentiated by adipose-derived stemcells harvested from transgenic mice

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