Differentiation of adult human bone marrow mesenchymal stem cells into Schwann-like cells in vitro

YANG Li-ye; ZHENG Jia-kun; WANG Chao-yang; LI Wen-yu

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Differentiation of adult human bone marrow mesenchymal stem cells into Schwann-like cells in vitro

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Objective: To investigate the differentiative capability of adult human bone marrow mesenchymal stem cells (BMSCs) into Schwann-like cells. Methods: Bone marrows were aspirated from healthy donors and mononuclear cells were separated by Percoll lymphocytes separation liquid (1.073 g/ml) with centrifugation, cells were cultured in DMEM/F12 (1:1) medium containing 10% fetal bovine serum (FBS), 20 ng/ml epidermal growth factor (EGF) and 20 ng/ml basic fibroblast growth factor (bFGF). Cells of passage 1 were identified with immunocytochemistry. Conclusions: Bone marrow contains the stem cells with the ability of differentiating into Schwann-like cells, which may represent an alternative stem cell sources for neural transplantation.

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《中华创伤杂志（英文版）》 |2005年第2期|77-80|共4页
作者
YANG Li-ye; ZHENG Jia-kun; WANG Chao-yang; LI Wen-yu;
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作者单位

Department of Neurosurgery, Chaozhou Central Hospital, Guangdong 521021, China;

Department of Neurosurgery, Chaozhou Central Hospital, Guangdong 521021, China;

Department of Neurosurgery, Chaozhou Central Hospital, Guangdong 521021, China;

Department of Neurosurgery, Chaozhou Central Hospital, Guangdong 521021, China;

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正文语种 chi
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关键词
Mesenchymal stem cells; Bone marrow; Cell differentiation; Schwann cells;

机译：间充质干细胞;骨髓;细胞分化;施旺细胞;

Differentiation of adult human bone marrow mesenchymal stem cells into Schwann-like cells in vitro

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