Effect of Shenfu injection on nuclear factor-kB during myocardial ischemia/reperfusion injury in rats

ZHANG Ben-jing; WANA Yan-lin; WANG Cheng-yao; KE Jian-juan

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Effect of Shenfu injection on nuclear factor-kB during myocardial ischemia/reperfusion injury in rats

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Objective: To investigate effects of Shenfu injection on the concentrations of plasma tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6), activity of Nuclear Factor kappa B (NF-κB) and heart tissue ultrastructure during myocardial ischemia/reperfusion (I/R) injury in rats and its potential mechanism.Methods: Myocardial ischemia/reperfusion (I/R) was produced by ligation and release of the left anterior descending coronary artery. Ischemia lasted for 30 min and reperfusion for 60 min. Twenty-four healthy male SD rats weighing 230-280 g were randomly divided into three groups (n=8, each): Group I (Sham-operation group); Group II (I/R group); Group III (Shenfu group), in which Shenfu injection (10 ml/kg) was intraperitoneally injected 30 min before ischemia in animals with I/R. The plasma concentrations of IL-6 and TNF-α were measured by ELISA, and the heart was harvested for determination of NF-κB levels by Ecl-western blot analysis. Electron microscopy was used to study its ultrastructure.Results: After reperfusion, NF-κB binding activity in myocardial nuclei and the plasma concentrations of IL-6 and TNF-α were significantly increased in Group II, compared with Group I (P<0.01), and they were markedly reduced in Group III, compared with Group II (P<0.01). In addition, electron microscopic examination showed more serious injury of the myocardium ultrastructure in Group II, while in Group III the myocardial ultrastructure was similar to normal state.Conclusions: Shenfu injection inhibits NF-κB activity in I/R myocardium and leads to down-regulation of proinflammatory cytokine expression, which might be one of the molecular mechanisms of Shenfu injection in cardioprotection.

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《中华创伤杂志（英文版）》 |2005年第4期|200-204|共5页
作者
ZHANG Ben-jing; WANA Yan-lin; WANG Cheng-yao; KE Jian-juan;
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作者单位

Department of Anesthesiology, Zhongnan Hospital, Wuhan University, Wuhan 430071, China;

Department of Anesthesiology, Zhongnan Hospital, Wuhan University, Wuhan 430071, China;

Department of Anesthesiology, Zhongnan Hospital, Wuhan University, Wuhan 430071, China;

Department of Anesthesiology, Zhongnan Hospital, Wuhan University, Wuhan 430071, China;

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原文格式 PDF
正文语种 chi
中图分类外科学;
关键词
Ischemia; Reperfusion; NF-kappa B; Tumor necrosis factor-alpha; Receptors,Interleukin-6; Shenfu injection;

机译：缺血;再灌注;NF-κB;肿瘤坏死因子-α;白细胞介素6受体;参附注射液;

Effect of Shenfu injection on nuclear factor-kB during myocardial ischemia/reperfusion injury in rats

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