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蕤核染色体数目及核型分析

         

摘要

The seed germination method of Prinsepia uniflora Batal was optimized. The chromosome number and the karyotype were investigated with conventional plant root tip squashing method. The results showed that the best germination method was shell breaking and sand burial of the seed. The seed germination rate was up to 61%. The chromosome number was 28, with karyotype formula K(2n)= 2x=28= 28m. The change of ration of chromosome length(L/S)was between 1.042~1.600, with an average ration of 1.184. The karyotype type was 1A.%以蕤核为实验材料,通过不同的处理方式探索蕤核种子的最佳发芽方式,根尖压片法鉴定蕤核的染色体数目和核型.结果表明:蕤核的最佳发芽方式为破壳沙埋处理,发芽率可达61%;染色体数目为28,其核型公式为2n=2x=28=28m,臂比值变化在1.042~1.600之间,平均臂比为1.184.核型类别为1A.

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