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首页> 中文期刊> 《热带作物学报》 >中美重要甘蔗种质SSR遗传多样性比较

中美重要甘蔗种质SSR遗传多样性比较

         
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摘要

美国种质是中国甘蔗育种中重要的亲本材料,在中国甘蔗育种中具有重要的地位.本试验应用18对SSR引物对来自美国的12份种质和中国选育的36份种质进行分析,比较中、美甘蔗种质间的遗传多样性差异.48份材料在18对引物上共检测到257条多态性条带,平均每对引物上的多态性条带数为16.8,试验材料间的Jaccard 遗传相似性系数介于0.3 913～0.7 246之间,平均为0.5 383.以Jaccard 遗传相似性系数进行UPGMA聚类,在阀值约0.5时,48份材料可划分为3类,第一类包括34份中国材料和4份美国材料;第二类包括8份美国材料;第三类为具有热带种越南牛蔗血缘的两份中国材料.分析结果表明,美国种质和中国种质间具明显的遗传差异,继续加强美国种质的引进和应用有助于拓宽中国甘蔗品种的遗传基础;越南牛蔗的后代和其他材料之间具有明显的遗传差异,育种时应加强热带种的创新利用.%Sugarcane(Saccharum spp.)germplasm introduced from U.S.A. was an important type of parental materials and played a key role in Chinese sugaracane breeding program. In this study, genetic diversity of 48 accessions including 36 from China and 12 from U.S.A. was assessed by SSR markers. In 18 loci, a total of 257 alleles were detected with an average of 16.8. For most loci, there was no obvious difference in the allele numbers between the Chinese and American accessions. Difference was only found at several loci such as SSCIR36,SSCIR43, SSCIR32. Jaccard’s similarity coefficient among the accessions was ranged from 0.3913 to 0.742 6 and that among the accessions from the same country was higher than that among the accessions between the two countries. Clustering based on Jaccard's coefficient using UPGMA showed the total 48 accessions could be clustered into three groups. Thirty-four Chinese accessions and four America accessions were clustered into a group. Eight American accessions were clustered into the second group and two accessions from crosses of “Yuenan niuzhe” (Saccharum officinarum L.)and Saccharum spontaneaum L. into the third group. The clustering analysis showed there were significant differences in the genetic base of the accessions from the two countries. It would be helpful to improve the genetic diversity of sugarcane varieties by using more American accessions in Chinese breeding program. It was also shown that there was considerable genetic difference between the offsprings from＂ Yuenan niuzhe＂ Saccharum officinarum L.)and other cultivars. It is recommended to use more germplasm of Saccharum offtcinarum L. in future breeding program.

著录项

  • 来源
    《热带作物学报》 |2011年第1期|99-104|共6页
  • 作者

    齐永文; 劳方业; 张垂明; 樊丽娜; 何慧怡; 刘少谋; 李奇伟; 邓海华;

  • 作者单位

    广州甘蔗糖业研究所,广东省甘蔗改良与生物炼制重点实验室,广东广州510316;

    广州甘蔗糖业研究所,广东省甘蔗改良与生物炼制重点实验室,广东广州510316;

    广州甘蔗糖业研究所,广东省甘蔗改良与生物炼制重点实验室,广东广州510316;

    广州甘蔗糖业研究所,广东省甘蔗改良与生物炼制重点实验室,广东广州510316;

    广州甘蔗糖业研究所,广东省甘蔗改良与生物炼制重点实验室,广东广州510316;

    广州甘蔗糖业研究所,广东省甘蔗改良与生物炼制重点实验室,广东广州510316;

    广州甘蔗糖业研究所,广东省甘蔗改良与生物炼制重点实验室,广东广州510316;

    广州甘蔗糖业研究所,广东省甘蔗改良与生物炼制重点实验室,广东广州510316;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 甘蔗;
  • 关键词

    甘蔗; 遗传多样性; SSR; 中国; 美国;

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