Objective To observe the optoacoustic imaging effect and safety of indocyanine green and liquid perfluorohexanes loaded nanoparticles contrast agents decorated with SDF-1 in the lymph node metastasis of rabbit with tongue cancer.Methods Preparation of the nanoparticles agent,PLGA shell,ICG and PFH kernel,decorated with SDF-1 by means of emulsification technique.To observe the optoacoustic imaging effect in the lymph node metastasis of rabbit with tongue cancer,the photoacoustic intensity of different contrast agents were compared at the same time.After 1 weeks of injection of contrast agent and,all the healthy rabbits underwent the physical examination,complete blood count,liver and renal function tests.During this period,the emotion state and vital signs of rabbits should be evaluated.The rabbits were killed after one month,and the pathological examination of the irradiated and non-irradiated skin,lung,liver and kidney were carried out.Results After the injection of indocyanine green and liquid perfluorohexanes loaded nanopartieles contrast agents decorated with SDF-1,photoacoustic imaging began to occur in 20 min and disappeared in 24 h.The photoacoustic imaging of the metastatic lymph node is significantly elevated.Little photoacoustic signal was found in the blank group.There was no significant difference in physical sign of the normal rabbits during the first month after intravenous injection of contrast agent.The histological examination of liver,lung,kidney and the skin of irradiated area was not significantly different from that of the normal rabbits.The effect of photoacoustic imaging of metastatic lymph nodes was significantly enhanced by indocyanine green and liquid perfluorohexanes loaded nanoparticles contrast agents decorated with SDF-1.Conclusions The results of this study indicate that the a gents is a safe,effective and inexpensive synergist.%目的 观察SDF-1所修饰的包裹吲哚菁绿和液态氟碳造影剂对于兔舌癌颈部转移淋巴结的光声成像效果及安全性检测.方法 采用乳溶剂挥发法制备出聚乳酸-羟基乙酸共聚物(PLGA)为载体,SDF-1为配体,吲哚菁绿(ICG)和液态氟碳(PFH)为内核的纳米粒,构建SDF-1所修饰的包裹吲哚菁绿和液态氟碳的纳米粒造影剂,检测患有舌癌并发生颈淋巴转移的新西兰大白兔的光声成像效果.健康的新西兰大白兔在注射造影剂后第1周和第3周,进行血常规,肝、肾功能检测并观察新西兰大白兔的精神、外貌等体征.1个月后处死动物,进行照射区域皮肤、肺脏、肝脏以及肾脏的病理检测.结果 注射SDF-1修饰的包裹吲哚菁绿和液态氟碳的造影剂后,20 min可见光声信号,24 h后完全消失,实验组较对照组有明显增强转移淋巴结成像效果,空白组未见明显光声信号.健康的兔子经静脉注入造影剂后1个月内,生命体征无明显异常.病理检查照射区域皮肤、肝、肺及肾脏组织学检测学与正常兔子无明显差异.结论 SDF-1所修饰的包裹吲哚菁绿和液态氟碳的纳米粒造影剂有明显增强转移淋巴结的光声成像效果,是一种安全、有效、价廉的光声成像增效剂.
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