We investigated the serotype,drug resistance features,molecular types,and homology of Salmonella in diarrhea in Tai'an City,and provided scientific evidences for warning,control and clinical treatment.The 31 Salmonella strains isolated from stool specimens of diarrhea in Tai'an were identified by serotyping.Broth dilution technique was used to test the sensitivity of 31 strains of Salmonella to 16 antibiotics.Pulsed field gel-electrophoresis (PFGE) was used for molecular typing and cluster analysis.Results showed that 31 strains of Salmonella were divided into 10 kinds of serotype.S.enteritidis (11/31) was the predominant serotype.All 31 strains of Salmonella were sensitive to cefoxitin and imipenem.The drug resistance rates of the 31 strains of Salmonella were 77.42 ％ (24/31) to sulfafurazole.The 31 strains of Salmonella were divided into 17 patterns by PFGE,with 100％ similarity for each pattern.All the results suggest that there are more serious problems in multiple drug resistance.The genetic variation of S.enteritidis is relatively conservative.Early warning and control on S.enteritidis are exposed to reinforce.%目的 分离鉴定泰安市腹泻病沙门菌的血清型分布、耐药性及PFGE分子分型和同源性特点.方法 对分离自泰安市腹泻病人的粪便标本中的31株沙门菌进行生化鉴定和血清分型,采用微量肉汤稀释法对其进行16种抗生素敏感试验,利用PFGE进行分子分型及聚类分析.结果 31株沙门菌分属于10种血清型,肠炎沙门菌占35.5％(11/31)为优势血清型;31株沙门菌对头孢西丁和亚胺培南100％敏感,对磺胺异噁唑的耐药率为77.42％(24/31);31株沙门菌按照100％的相似度可分为17个PFGE型.结论 药敏显示所检测样本存在较为严重的多重耐药性,肠炎沙门菌遗传变异在该地区相对较为保守,且存在散发流行的可能,提示应做好肠炎沙门菌传染病的早期预警及防控.
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