In order to figure out phylogenetic relationship and genetic diversity of different geographical populations,genetic analyses of Aedes albopictus were performed based on mitochondrial gene COI.Based on samples collected from most distribution regions in China,mitochondrial gene Cytochrome C Oxidase Subunit I was obtained through PCR and DNA sequence.Together with some COI sequences downloaded from GenBank,60 COI sequences with the final length of 598 bp were used for subsequent analyses.Results showed that there was no obvious divergence according to phylogenetic analyse,all sequences were clustered together in Maximum Likelihood tree.Sixteen haplotypes were detected,and four of them shared haplotypes.Haplotype diversity (Hd) was 0.737,nucleotide diversity (π) was 0.20 ％.Population genetic differentiation analyses demon strated that Hainan population showed obvious divergences.In the network of haplotypes,H1 and H6 was found to be the primary haplotypes,and they formed two radical centers.All these results indicate that A.albopictus populations of China are expanding presently,and Hainan population become differential with other geographical populations,which probably attribute to geographical isolations.%目的 探讨白纹伊蚊不同地理种群间的进化关系和遗传分化情况,为白纹伊蚊防制和蚊媒病防控提供基础资料.方法 在广泛采集样品的基础上,通过PCR扩增、测序获取线粒体基因COI片段,并从GenBank下载了部分序列,比对、剪切后的598 bp用于后续分析.结果 系统发育分析的结果表明所有白纹伊蚊的COI序列聚成一支,没有明显的遗传差异;60条COI序列分属于19个单倍型,其中4个为共享单倍型;单倍型多样性(Hd)为0.737,核苷酸多样性(π)为0.20％;海南的白纹伊蚊种群与绝大部分地理种群间出现了明显的分化(P＜0.05);H1和H6形成了2个辐射中心,是较为原始的单倍型.结论 我国的白纹伊蚊种群正处于扩张的趋势,海南的白纹伊蚊种群与其它地理种群间出现了明显分化.
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