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首页> 中文期刊> 《中国人兽共患病学报》 >云南西部地区家畜动物感染巴贝虫的分子流行病学调查

云南西部地区家畜动物感染巴贝虫的分子流行病学调查

         
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摘要

目的 了解云南省西部地区家畜动物感染巴贝虫的状况,为制定防治措施提供依据.方法 在云南省西部地区12个县(市)采集家畜动物抗凝血液1 073份(牛血274份、羊血395份、犬血354份,马血33份,驴血17份).提取基因组DNA,运用PCR检测巴贝虫18S rRNA 1 600 bp长片段,通过基因序列比对及系统进化分析进行巴贝虫定种.结果 共检出巴贝虫阳性样本50份,分属5种巴贝虫,总阳性率4.66％.其中牛血中检出11份阳性(4.01％),4份为牛巴贝虫(B.bovis),7份为双芽巴贝虫(B.bigemina).羊血中检出38份阳性(9.62％),37份为奥氏巴贝虫样寄生虫(B.odocoilei-like parasites),1份为狍巴贝虫样寄生虫(B.capreoli-like parasites);犬血中检出阳性1份(0.28％),为韦氏巴贝虫(B.vogelli).马和驴未检出阳性.结论 云南省西部部分县市的家畜动物存在多种巴贝虫感染,给当地畜牧业的发展和人类的健康造成一定的危害,需加强防范,当地人群感染情况值得进一步调查.%The objective of this study was to investigate Babesia infection among domestic animals in Western Yunnan Province and provide scientific evidence for developing control measures.A total of 1 073 domestic animals blood samples (274cattle,395 sheep,354 dogs,33 horses and 17 donkeys) were collected in 12 counties in Western Yunnan Province.Genomic DNA was extracted and a near full-length 18S rRNA gene sequence of Babesia was amplified by using nested PCR.Babesia species was identified by Blast program and phylogenetic tree.It was indicated that 50 samples were infected with Babesia,belonging to 5 species and with the infection rate of 4.66％.Among 274 cattle blood samples,11 were infected with Babesia (4.01％).Four of them were Babesia bovis and seven of them were Babesia bigemina.Among 395 sheep blood samples,38 were infected with Babesia (9.62 ％),37 of them were Babesia odocoilei-like parasites and 1 of them was Babesia capreoli-like parasites.Horses and donkeys were negative.In conclusion,domestic animals in Western Yunnan Province are infected with many kinds of Babesia,which threaten stock raising development and human health.It is necessary to strengthen prevention of babesiosis and investigate infection rate of babesiosis in human.

著录项

  • 来源
    《中国人兽共患病学报》 |2017年第12期|1125-1130|共6页
  • 作者

    黄涛华; 高子厚; 蒋宝贵; 赵文红; 苏超; 贾娜; 张青; 江佳富; 杜春红;

  • 作者单位

    云南省地方病防治所,大理671000;

    大理大学公共卫生学院,大理671000;

    云南省地方病防治所,大理671000;

    军事医学科学院微生物流行病研究所病原微生物安全国家重点实验室,北京 100071;

    云南省地方病防治所,大理671000;

    云南省地方病防治所,大理671000;

    军事医学科学院微生物流行病研究所病原微生物安全国家重点实验室,北京 100071;

    云南省地方病防治所,大理671000;

    军事医学科学院微生物流行病研究所病原微生物安全国家重点实验室,北京 100071;

    云南省地方病防治所,大理671000;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 医学原虫学;
  • 关键词

    云南省; 家畜动物; 巴贝虫; 调查;

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