Effects of Matricaria chamomilla Extract on Growth and Maturation of Isolated Mouse Ovarian Follicles in a Three-dimensional Culture System

Hamed Shoorei; Sayed Haidar Abbas Raza; Arash Khaki; Nava Ainehchi; Moloud Tahmasebi; Giti Seyedghiasi; Ziba Ghoreishi; Majid Shokoohi; Amir Afshin Khaki

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Effects of Matricaria chamomilla Extract on Growth and Maturation of Isolated Mouse Ovarian Follicles in a Three-dimensional Culture System

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Background:The aim of this study was to design and assess the effects of hydroalcoholic extract of Matricaria chamomilla (MC) on preantral follicle culture of mouse ovaries in a three-dimensional culture system.Methods:Isolated preantral follicles were randomly divided into three main groups:the control group containing 10％ fetal bovine serum without MC extract (G 1),the first experimental group supplemented with 25 μg/ml hydroalcoholic extract of chamomile (G2),and the second experimental group supplemented with 50 μg/ml hydroalcoholic extract of chamomile (G3).Results:After 12 days of culture,the survival rate (P ＜ 0.05),antrum formation (P ＜ 0.01),metaphase two oocytes (P ＜ 0.01),and the expression ofPCNA (P ＜ 0.05) and FSHR (P ＜ 0.05) genes significantly decreased in G3 as compared with G1.On the other hand,at the last day of culture (day 12),the mean diameter of follicles cultured in the medium which was supplemented with 50 μg/ml hydroalcoholic extract of chamomile significantly decreased as compared with the G1 (P ＜ 0.05).In addition,the levels of progesterone and dehydroepiandrosterone hormones significantly increased in the medium of G3 relative to G1 (P ＜ 0.01),while in the medium of G 1,the level of 17β-estradiol was significantly higher than that of other groups (P ＜ 0.01).Reactive oxygen species levels of metaphase Ⅱ oocytes were significantly decreased in G2 as compared with G1 (P ＜ 0.01).Conclusion:Adding chamomile extract to culture media appeared to decrease follicular function and development.

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《中华医学杂志（英文版）》 |2018年第2期|218-225|共8页
作者
Hamed Shoorei; Sayed Haidar Abbas Raza; Arash Khaki; Nava Ainehchi; Moloud Tahmasebi; Giti Seyedghiasi; Ziba Ghoreishi; Majid Shokoohi; Amir Afshin Khaki;
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Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran;

Department of Biology, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China;

Department of Pathology, Tabriz Branch, Islamic Azad University, Tabriz, Iran;

Women's Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;

Department of Anatomical Sciences, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran;

Women's Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;

Department of Nursing, Faculty of Paramedical, Mashhad University of Medical Sciences, Mashhad, Iran;

Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran;

Women's Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;

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Effects of Matricaria chamomilla Extract on Growth and Maturation of Isolated Mouse Ovarian Follicles in a Three-dimensional Culture System

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