Treponema pallidum-specific antibody expression for the diagnosis of different stages of syphilis

SUN Ran; LAI Di-hui; REN Rong-xin; LIAN Shi; ZHANG Hai-ping

摘要

Background Tp15,Tp17,Tp45,and Tp47 are outer-membrane proteins found in Treponema pallidum,the etiologic agent of syphilis.These proteins are potent antigens and are potential markers for the serological detection of syphilis.The present study analyzed antibodies to these protein antigens (TP-IgM and TP-IgG) in human serum and investigated the expression of these antibodies during different stages of syphilis.Methods Serum samples were collected from 69 subjects (male 45,female 24) diagnosed with syphilis and analyzed by Western blotting for the expression of IgM and IgG against the four protein antigens.Expression levels of the target antibodies were compared during the same stage of syphilis as well as between different stages of this disease.Results In subjects with primary syphilis,the positive rate of Tp45 IgM was higher than that of other TP-IgM.Tp15 IgM was detected only in subjects with tertiary syphilis.Similarly,the seroprevalence of Tp45 IgG in primary syphilis was higher than for other TP-IgG No target TP-IgM was detected in subjects with latent syphilis.In subjects with secondary syphilis,the expression level of Tp15 IgG (138.73±20.16) was higher than for other target TP-IgG In subjects with tertiary syphilis,all target TP-IgG were detected.In subjects with tertiary or latent syphilis,the expression levels of Tp45 IgG (121.33±11.04 and 110.10±40.19,respectively) were higher than those of other target TP-IgG.The expression levels of all Tp-IgM were similar before or after anti-syphilis treatment.In comparison,the expression levels of all TP-IgG decreased compared with the pre-treatment levels,and this decrease was statistically significant (both P ＜0.05) for Tp17 IgG and Tp47 IgG.Conclusions After Treponema pallidum infection,Tp45 IgM appeared first and Tp15 IgM occurred during later stages.The positive rates of all TP-IgG increased with the duration of this disease.Anti-syphilis treatment reduced the expression levels of Tp17 IgG and Tp47 IgG.Larger-scale studies are required to further validate the value of Tp15,Tp17,Tp45,and Tp47 as markers for the early detection of primary and latent syphilis.

机译：背景Tp15，Tp17，Tp45和Tp47是梅毒螺旋体中的外膜蛋白，是梅毒的病原体。这些蛋白是有效的抗原，是梅毒血清学检测的潜在标志物。本研究分析了针对这些蛋白抗原的抗体方法从人血清中检测TP-IgM和TP-IgG，并研究这些抗体在梅毒不同阶段的表达。方法从69例诊断为梅毒的受试者（男性45岁，女性24岁）中收集血清样品，并通过Western blotting分析IgM和IgG对四种蛋白质抗原的表达。比较梅毒在同一阶段以及该疾病不同阶段之间靶抗体的表达水平。结果在原发性梅毒患者中，Tp45 IgM的阳性率更高与其他TP-IgM相比，Tp15 IgM仅在三期梅毒患者中被检测到。同样，原发性梅毒中Tp45 IgG的血清阳性率较高。对于其他TP-IgG，在潜伏梅毒患者中未检测到目标TP-IgM。在继发性梅毒患者中，Tp15 IgG的表达水平（138.73±20.16）高于其他目标TP-IgG在三期梅毒患者中在所有三级或潜在梅毒患者中，Tp45 IgG的表达水平（分别为121.33±11.04和110.10±40.19）高于其他目标TP-IgG。 Tp-IgM在梅毒治疗前后相似。相比之下，所有TP-IgG的表达水平均比治疗前降低，而Tp17 IgG和Tp47的表达均具有统计学意义（P ＜0.05）。结论梅毒螺旋体感染后首先出现Tp45 IgM，随后出现Tp15 IgM，所有TP-IgG的阳性率均随着病程的延长而增加，抗梅毒治疗降低了Tp17 IgG和Tp47 IgG的表达水平。。较大的需要进行文献研究以进一步验证Tp15，Tp17，Tp45和Tp47作为早期发现梅毒和潜伏梅毒的标志物的价值。

Treponema pallidum-specific antibody expression for the diagnosis of different stages of syphilis

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