An imaging study using laminin peptide 99mTc-YIGSR in mice bearing Ehrlich ascites tumour

HU Jia; ZHANG Yong-xue; LAN Xiao-li; QIN Guang-ming; ZHANG Jun; HU Zhi-hong

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An imaging study using laminin peptide 99mTc-YIGSR in mice bearing Ehrlich ascites tumour

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Background The YIGSR is a pentapeptide, from the laminin-1 of the β1 chain, which can mediate cell adhesion and bind the 67 kD laminin receptor. The purpose is to evaluate the usefulness of 99mTc-YIGSR, a novel tumour radiotracer, in the receptor imaging of Ehrlich ascites tumour.Methods Using S-Acetly-NH3-MAG3 as chelate, YIGSR, a pentapeptide from laminin, was tagged with 99mTc. 99mTc-YIGSR was detected in the tumour group bearing Ehrlich ascites tumour and blocked group. Tumour, normal, inflammatory and blocked groups were imaged. Results Through reverse phase Sep-Pak C18 chromatogram, it was revealed that YIGSR could conjugate with S-Acetly-NH3-MAG3, and be radiolabelled at room temperature and neutral pH with a radiolabelling yield of 62%, and of 4% without chelate. 99mTc-YIGSR was rapidly cleared from kidney, then liver. The imaging findings showed tumour tissue accumulated initial radioactivity at fifteen minutes after injection in the tumour group, and the uptake increased to peak at three hours with a tumour/muscle ratio (T/M) of 11.36, then cleared slowly to a T/M of 7.50 at eight hours. The tumour uptake of radiotracer in blocked group was significantly lower with T/M of 4.61 at three hours and 0.89 at eight hours. The T/M was only 3.72 at three hours and 1.29 at eight hours after injection in inflammatory group. Compared with inflammatory group and control obstructive group, the ratio of T/M in tumour group was significantly different (P<0.001). Conclusions Using S-Acetly-NH3-MAG3, we radiolabelled YIGSR with 99mTc. 99mTc-YIGSR possesses many merits of tumour imaging: rapid visualization, high sensitivity and specificity, and satisfactory target/nontarget ratio. Our data suggest 99mTc-YIGSR is a promising tumour radiotracer.

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《中华医学杂志（英文版）》 |2005年第9期|753-758|共6页
作者
HU Jia; ZHANG Yong-xue; LAN Xiao-li; QIN Guang-ming; ZHANG Jun; HU Zhi-hong;
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作者单位

Department of Nuclear Medicine,Union Hospital,Huazhong University of Science and Technology,Wuhan 430022,China;

Department of Nuclear Medicine,Union Hospital,Huazhong University of Science and Technology,Wuhan 430022,China;

Department of Nuclear Medicine,Union Hospital,Huazhong University of Science and Technology,Wuhan 430022,China;

Department of Nuclear Medicine,Shenzhen Second People's Hospital,Shenzhen 518035,China;

Department of Nuclear Medicine,Union Hospital,Huazhong University of Science and Technology,Wuhan 430022,China;

Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan 430074,China;

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原文格式 PDF
正文语种 chi
中图分类肿瘤学;
关键词
radionuclide imaging; 99mTc-YIGSR; laminin receptors;

机译：放射性核素显像;99mTc-YIGSR;层粘连蛋白受体;

An imaging study using laminin peptide 99mTc-YIGSR in mice bearing Ehrlich ascites tumour

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