Effects of (-)-epigallocatechin-3-gallate on expression of matrix metalloproteinase-1 and tissue inhibitor of metalloproteinase-1 in fibroblasts irradiated with ultraviolet A

宋秀祖; 夏济平; 毕志刚

首页> 中文期刊> 《中华医学杂志（英文版）》 >Effects of (-)-epigallocatechin-3-gallate on expression of matrix metalloproteinase-1 and tissue inhibitor of metalloproteinase-1 in fibroblasts irradiated with ultraviolet A

Effects of (-)-epigallocatechin-3-gallate on expression of matrix metalloproteinase-1 and tissue inhibitor of metalloproteinase-1 in fibroblasts irradiated with ultraviolet A

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Background It is known that ultraviolet irradiation can affect cellular function through a number of signaling pathways. (-)-epigallocatechin-3-gallate (EGCG) is the major effective component in green tea and can offer protection from ultraviolet-induced damage. In this study, we investigated the protective mechanism of EGCG on human dermal fibroblasts damaged by ultraviolet A (UVA) in vitro.Methods Transcription factor Jun protein levels were measured by Western blot. Matrix metalloproteinase 1 (MMP-1) and tissue inhibitor of metalloproteinase-1 (TIMP-1) mRNA were studied by reverse transcription-polymerase chain reaction (RT-PCR) analysis in conjunction with computer-assisted image analysis. MMP-1 and TIMP-1 proteins were quantified by enzyme-linked immunosorbent assay (ELISA).Results EGCG decreased transcription activity of Jun protein after induction by UVA. Both the mRNA and protein levels of MMP-1 were increased by UVA irradiation, while no significant changes were observed in TIMP-1 levels. The ratio of MMP-1 to TIMP-1 showed statistically significant differences compared with the control. EGCG decreased the ratio of MMP-1 to TIMP-1 by inhibiting UVA-induced MMP-1 expression (P<0.05).Conclusion EGCG can protect human fibroblasts against UVA damage by downregulating the transcription activity of Jun protein and the expression of MMP-1. The ratio of MMP-1 to TIMP-1, rather than the levels of MMP-1 or TIMP-1 alone, may play a significant role in human skin photodamage.

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《中华医学杂志（英文版）》 |2004年第12期|1838-1841|共4页
作者
宋秀祖; 夏济平; 毕志刚;
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Department of Dermatology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China;

Department of Dermatology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China;

Department of Dermatology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China;

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原文格式 PDF
正文语种 chi
中图分类基础医学;
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ultraviolet A; fibroblasts; (-)-epigallocatechin-3-gallate; matrix metalloproteinase 1; tissue inhibitor of metalloproteinase-1;

机译：紫外线A;成纤维细胞;（-）-表没食子儿茶素-3-没食子酸酯;基质金属蛋白酶1;金属蛋白酶-1的组织抑制剂;

Effects of (-)-epigallocatechin-3-gallate on expression of matrix metalloproteinase-1 and tissue inhibitor of metalloproteinase-1 in fibroblasts irradiated with ultraviolet A

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