False Human Immunodeficiency Virus Test Results Associated with Rheumatoid Factors in Rheumatoid Arthritis

Yun-chun Li; Fan Yang; Xiao-yun Ji; Zhong-jun Fang; Jun Liu; Yue Wang

首页> 外文期刊>中国医学科学杂志（英文版） >False Human Immunodeficiency Virus Test Results Associated with Rheumatoid Factors in Rheumatoid Arthritis

【24h】

False Human Immunodeficiency Virus Test Results Associated with Rheumatoid Factors in Rheumatoid Arthritis

机译：类风湿关节炎中与类风湿因子相关的错误的人类免疫缺陷病毒测试结果

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

页面导航

摘要
著录项
相关主题

摘要

Objective To investigate if immunological factors associated with rheumatoid arthritis (RA) affect the result of human immunodeficiency virus (HIV) screening by electrochemiluminescence immunoassay (ECLIA) and enzyme-linked immunosorbent assay (ELISA). Methods 100 RA cases were enrolled from January 2012 to February 2013 into this study. HIV screening was conducted with ECLIA detecting both HIV-1 p24 antigen, HIV-1 and HIV-2 antibodies, with ELISA and colloidal gold method detecting HIV-1 and HIV-2 antibodies. The samples producing positive results were submitted to the Center for Disease Control for confirmation using Western blotting method. The antibody titers of rheumatoid factors (RF) including RF-IgG, RF-IgM, RF-IgA, and CCP-IgG were analyzed by ELISA. Results The HIV positive-rate determined by ECLIA was significantly higher than that by ELISA and colloidal gold method (P<0.01). The false-positive rate of HIV screening was associated with antibody titers of RF-IgG, RF-IgM, RF-IgA, and CCP-IgG in RA (P<0.01). Conclusion Immunological factors, including RF and anti-CCP antibody, may influence the screening of HIV by ECLIA, producing false-positive result.

机译：目的探讨类风湿关节炎（RA）相关的免疫学因素是否通过电化学发光免疫测定（ECLIA）和酶联免疫吸附测定（ELISA）影响人免疫缺陷病毒（HIV）筛查的结果。方法自2012年1月至2013年2月，共纳入100例RA患者。通过ECLIA检测HIV-1 p24抗原，HIV-1和HIV-2抗体，并通过ELISA和胶体金法检测HIV-1和HIV-2抗体进行HIV筛查。产生阳性结果的样品将提交给疾病控制中心，以使用蛋白质印迹法进行确认。通过ELISA分析包括RF-IgG，RF-IgM，RF-IgA和CCP-IgG在内的类风湿因子（RF）的抗体效价。结果ECLIA测定的HIV阳性率明显高于ELISA和胶体金法检测的HIV阳性率（P <0.01）。 HIV筛查的假阳性率与RA中RF-IgG，RF-IgM，RF-IgA和CCP-IgG的抗体滴度有关（P <0.01）。结论结论免疫因素，包括RF和抗CCP抗体，可能会影响ECLIA对HIV的筛查，从而产生假阳性结果。

著录项

来源
《中国医学科学杂志（英文版）》 |2014年第2期|103-106|共4页
作者
Yun-chun Li; Fan Yang; Xiao-yun Ji; Zhong-jun Fang; Jun Liu; Yue Wang;
展开▼
作者单位

Department of Laboratory Center, Shanghai Guanghua Hospital of Integrative Medicine, Shanghai 200052, China;

Department of Laboratory Center, Shanghai Guanghua Hospital of Integrative Medicine, Shanghai 200052, China;

Department of Laboratory Center, Shanghai Guanghua Hospital of Integrative Medicine, Shanghai 200052, China;

Department of Laboratory Center, Shanghai Guanghua Hospital of Integrative Medicine, Shanghai 200052, China;

Department of Laboratory Center, Shanghai Guanghua Hospital of Integrative Medicine, Shanghai 200052, China;

Department of Laboratory Center, Shanghai Guanghua Hospital of Integrative Medicine, Shanghai 200052, China;

展开▼
收录信息中国科学引文数据库(CSCD);中国科技论文与引文数据库(CSTPCD);
原文格式 PDF
正文语种 eng
中图分类
关键词

False Human Immunodeficiency Virus Test Results Associated with Rheumatoid Factors in Rheumatoid Arthritis

摘要

著录项

相关主题

期刊订阅