Serum Levels of Interleukin-1 Beta, Interleukin-6 and Melatonin over Summer and Winter in Kidney Deficiency Syndrome in Bizheng Rats

Miao Zhang; Tong Wang; Huai-min Chen; Yan-qin Chen; Yang-chun Deng; Ya-tian Li

摘要

Objective To observe the seasonal changes in serum levels of interleukin-1 beta (IL-1β), interleukin-6 (IL-6) and melatonin (MT) in Bizheng rat model, and explore the relationship between MT and the pathogenesis of rheumatoid arthritis. Methods One hundred and sixty Sprague-Dawley rats were randomly divided into four groups in summer (n=80) and winter (n=80) respectively:normal group, collagen-induced arthritis (CIA) model group, operation group, and sham-operation group (n=20 in each group). The CIA model group was injected with collagen emulsion at the base of the tail to induce arthritis. The rats in the operation group received pineal gland resection, and 7 days after the first operation, underwent testectomy or oophorectomy. The rats in the sham-operation group were operated to ligature the sagittal sinus, without extracting the pineal gland. After the operations, the operation group and the sham-operation group both were immunized as the CIA group was.The serum levels of IL-1β, IL-6 and MT in different groups were measured by radioimmunoassay. Results Compared with the normal group, the serum levels of IL-1βand IL-6 increased in the CIA model, operation, and sham-operation groups both in summer and in winter (IL-1βin summer, P=0.008, P<0.01, P=0.012; IL-1β in winter, P=0.019, P<0.01, P=0.027; IL-6 in summer, P=0.028, P<0.01, P=0.024;IL-6 in winter, P=0.006, P<0.01, P=0.008). In the operation group, the serum levels of IL-1βand IL-6 in winter were higher than in summer, but with no statistically significant differences (P=0.844, 0.679). Compared with the normal group, the serum level of MT significantly increased in summer and winter in both the CIA model group (P=0.002, 0.008) and the sham-operation group (P=0.003, 0.007), while significantly decreased in the operation group (P=0.023, 0.003). There was no significant difference in MT level in the operation group between summer and winter (P=0.947). Conclusions The increase of serum levels of IL-1β and IL-6 may exacerbate the inflammatory reaction and cause a more severe condition in the rheumatoid arthritis. The concentrations of IL-1β, IL-6, and MT correspond with the change of seasons, confirming that there are connections between nature and human body.

机译：目的观察Bizheng大鼠模型中血清IL-1β（IL-1β），IL-6（IL-6）和褪黑激素（MT）水平的季节性变化，探讨MT与类风湿关节炎发病机制的关系。。方法将160只Sprague-Dawley大鼠在夏季（n = 80）和冬季（n = 80）随机分为四组：正常组，胶原诱发的关节炎（CIA）模型组，手术组，假手术组（每组n = 20）。 CIA模型组在尾巴根部注射胶原乳剂以诱发关节炎。手术组的大鼠接受了松果体切除术，并且在第一次手术后7天进行了睾丸切除术或卵巢切除术。假手术组的大鼠在不提取松果体的情况下结扎矢状窦。手术后，手术组和假手术组均按CIA组免疫。用放射免疫法测定不同组的血清IL-1β，IL-6和MT水平。结果CIA模型组，手术组和假手术组的血清IL-1β和IL-6水平在夏季和冬季均高于正常组（夏季IL-1β，P = 0.008，P <0.01，P = 0.012;冬季IL-1β，P = 0.019，P <0.01，P = 0.027;夏季IL-6，P = 0.028，P <0.01，P = 0.024;冬季IL-6，P = 0.006，P <0.01，P = 0.008）。手术组冬季血清IL-1β和IL-6水平高于夏季，但差异无统计学意义（P = 0.844，0.679）。与正常组相比，CIA模型组（P = 0.002，0.008）和假手术组（P = 0.003，0.007）在夏季和冬季均显着升高MT的血清水平，而在手术中则明显降低组（P = 0.023，0.003）。夏季和冬季之间，手术组的MT水平无显着差异（P = 0.947）。结论结论IL-1β和IL-6的血清水平升高可能加剧类风湿关节炎的炎症反应，并导致更为严重的疾病。 IL-1β，IL-6和MT的浓度与季节变化相对应，这证明自然界与人体之间存在联系。

Serum Levels of Interleukin-1 Beta, Interleukin-6 and Melatonin over Summer and Winter in Kidney Deficiency Syndrome in Bizheng Rats

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