Real time detection of antibody-antigen interaction using a laser scanning confocal imaging-surface plasmon resonance system

张洪艳; 杨立泉; 孟岚; 聂家财; 宁廷银; 刘卫敏; 孙嘉宇; 汪鹏飞

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Real time detection of antibody-antigen interaction using a laser scanning confocal imaging-surface plasmon resonance system

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摘要

A laser scanning confocal imaging-surface plasmon resonance (LSCI-SPR) instrument integrated with a wavelength-dependent surface plasmon resonance (SPR) sensor and a laser scanning confocal microscopy (LSCM) is built to detect the bonding process of human IgG and fluorescent-labeled affinity purified antibodies in real time.The shifts of resonant wavelength at different reaction time stages are obtained by SPR,corresponding well with the changes of the fluorescence intensity collected by using LSCM.The instrument shows the merits of the combination and complementation of the SPR and LSCM,with such advantages as quantificational analysis,high spatial resolution and real time monitor,which are of great importance for practical applications in biosensor and life science.

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《中国物理：英文版》 |2012年第2期|224-228|共5页
作者
张洪艳; 杨立泉; 孟岚; 聂家财; 宁廷银; 刘卫敏; 孙嘉宇; 汪鹏飞;
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作者单位

Key Laboratory of Photochemical Conversion and Optoelectronic Materials;

Technical Institute of Physics and Chemistry;

Chinese Academy of Sciences;

Beijing 100190;

China;

Departments of Physics;

Beijing Normal University;

Beijing 100875;

China;

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原文格式 PDF
正文语种 chi
中图分类光检测技术;
关键词
激光扫描共聚焦显微镜; 表面等离子体共振; 共聚焦成像; 实时检测; 相互作用; 共振系统; 抗体; 激光共聚焦显微镜;

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Real time detection of antibody-antigen interaction using a laser scanning confocal imaging-surface plasmon resonance system

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