A New Method for Determining Dipole Moment Orientation of Single Molecules

WANG Chen; LIU Li; WANG Gui-Ying; XU Zhi-Zhan

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A New Method for Determining Dipole Moment Orientation of Single Molecules

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@@ A new imaging method is proposed to determine the three-dimensional dipole moment orientation of single fluorophore. Far-field microscopy can provide orientational information projected in the sample plane, while total internal reflection fluorescence microscopy (TIRFM) can offer the knowledge perpendicular to the surface because longitudinal electric-field components can be generated in total internal reflection geometry. By comparing fluorescence intensities measured with far-field epi-fluorescence microscopy and TIRFM, the exact information of single-fluorescent-molecule orientation is extracted. Detailed analysis of the method is given with a numerical example.

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《中国物理快报：英文版》 |2004年第5期|843-845|共3页
作者
WANG Chen; LIU Li; WANG Gui-Ying; XU Zhi-Zhan;
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Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800;

Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800;

Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800;

Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800;

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A New Method for Determining Dipole Moment Orientation of Single Molecules

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