目的：研究17β-雌二醇（17-βE2）对特定加力强度的雌性大鼠盆底肌成纤维细胞中基质金属蛋白酶-9（MMP-9）、金属蛋白酶组织抑制剂-1（TIMP-1）及β-联蛋白（β-catenin）的影响。方法原代培养雌性大鼠耻尾肌及周围筋膜并分离成纤维细胞，通过Flexercell 4000系统加载强度为10％、时间为12 h的机械应力，给予17-βE2干预，同时设置对照组（成纤维细胞组）以及机械应力组（强度为10％、时间为12 h的机械应力组）。荧光定量聚合酶链反应（PCR）测量各组MMP-9，TIMP-1及β-catenin mRNA的相对表达量。结果 RT-PCR结果显示，加力组与对照组相比，成纤维细胞中MMP-9 mRNA的相对表达量为2.690±0.020，高于对照组，差异有统计学意义（P＜0.05），TIMP-1 mRNA及β-catenin mRNA的相对表达量为0.370±0.010、0.430±0.010，低于对照组，差异有统计学意义（P＜0.05），17-βE2干预后，成纤维细胞中 MMP-9 mRNA相对表达量为1.523±0.068，高于对照组，低于加力组，差异有统计学意义（P＜0.05），TIMP-1 mRNA及β-catenin mRNA的相对表达量为0.600±0.010、0.630±0.010，低于对照组，高于加力组，差异有统计学意义（P＜0.05）。结论机械拉伸力可以使细胞形态和伸展方向发生改变，MMP-9表达增加，β-catenin及TIMP-1的表达下降，而17-βE2使得上述应力表现减弱。%Objective To investigate the effects of 17β-estradiol (17β-E2) on matrix metalloproteinase-9 (MMP-9), tissue inhibitor of metalloproteinase (TIMP-1) and β-catenin in the specific stressed pelvic myofibroblast of female rats. Methods Primary culture of pubococcygeus and peripheral fascia cells of female rats were segregated into fi-broblast. Then, the fibroblast were loaded with 10%mechanical stress for 12 h by Flexercell 4000 tensile loading sys-tem, and intervened with 17β-E2. Meanwhile, the control group (the fibroblast group) and mechanical stress group (10% stress-loaded for 12 h) were set up. The relative expression levels of MMP-9, TIMP-1 and β-catenin mRNA in each group were measured by fluorescent quantitative polymerase chain reaction (PCR). Results RT-PCR showed that, compared with the control group, higher relative expression level of MMP-9 mRNA (2.690±0.020), and lower rel-ative expression levels of TIMP-1mRNA and β-catenin mRNA (0.370 ±0.010 and 0.430 ±0.010) in fibroblasts were found in the mechanical stress group, with statistically significant differences (P<0.05). After intervened with 17β-E2, the relative expression level of MMP-9 mRNA of fibroblast (1.523±0.068) was higher than that in the control group and lower than that in the mechanical stress group, with statistical difference ( P<0.05);whereas the relative expression levels of TIMP-1 mRNA and β-catenin mRNA (0.600±0.010 and 0.630±0.010, respectively) were lower than those in the control group and higher than those in the mechanical stress group, with statistical differences ( P<0.05). Conclu-sion Mechanical stress can change the morphology and stretching direction of fibroblasts, increase MMP-9 expres-sion, and decrease β-catenin and TIMP-1 expressions. These effects can be attenuated by 17β-estradiol.
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