首页>
中文期刊>
《中国科学通报：英文版》
>Post-transcriptional gene silencing signal could move rapidly and bidirectionally in grafted Arabidopsis thaliana
Post-transcriptional gene silencing signal could move rapidly and bidirectionally in grafted Arabidopsis thaliana
展开▼
免费
页面导航
摘要
著录项
相似文献
相关主题
摘要
RNA interference (RNAi), one of the newly found ways for post-transcriptional gene si- lencing, has been widely used to investigate gene functions through transgenic methods for introducing an RNA silencing signal into plants. In the present study, we constructed a dexamethazone (DEX)-inducible RNAi binary vector harboring a specific sequence fragment (168-bp) homologous to KatB and KatC, two kinesin isoform genes of Arabidopsis, which were proved to result in the post-transcriptional gene si- lencing of KatB and KatC in DEX-induced transgenic plants. RT-PCR and Northern blot analysis on trans- genic homozygous Arabidopsis (termed as RNAi-type plants) showed that DEX inducement causes KatB and KatC mRNA degradation. With a simplified method, Arabidopsis grafting was effectively per- formed between RNAi-type and wild-type lines. The target gene mRNA levels were tested based on semi-quantitative RT-PCR. Our results demonstrateed that DEX-induced gene silencing signals could result in a reduction in KatB and KatC mRNA in the wild-type rootstocks or scions, indicating that silencing signals of RNAi could be transmitted bidirectionally across the graft junction whether RNAi-plants were scions or stocks. In contrast to the previously reported results on grafted tobacco, the transmission of post- tran- scriptional gene silencing signals caused by RNAi in grafted Arabidopsis is more effective than that in to- bacco.
展开▼
机译：a rapid and versatile combined DNa/RNa extraction protocol and its application to the analysis of a novel DNa marker set polymorphic between arabidopsis thaliana ecotypes Col-0 and Landsberg erecta
