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>Fast separation of intact proteins with high resolution under high sample loading using non-porous RPLC packings
Fast separation of intact proteins with high resolution under high sample loading using non-porous RPLC packings
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摘要
A new approach for separation of intact proteins with high resolution, high speed and high sample loading ("three highs") by non-porous packings in high performance liquid chromatography is presented.A chromatographic cake having larger diameter than its thickness is firstly employed to completely separate 1.0 and 40 μg of the mixture of 7 standard proteins within 1 min un-der conventional chromatographic conditions.Also, 0.5 mg of the mixture was almost completely separated within 1 min, accom-plishing the "three high" purpose.This research expands the application utilized for separation of intact proteins with non-porous packings.A smaller geometric size of chromatographic cake packed with much smaller particles of non-porous packings may improve results.
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