首页> 中文期刊> 《中国科学》 >Techniques for detecting protein-protein interactions in living cells:principles, limitations, and recent progress

Techniques for detecting protein-protein interactions in living cells:principles, limitations, and recent progress

         

摘要

Detecting protein-protein interactions(PPIs) provides fundamental information for understanding biochemical processes such as the transduction of signals from one cellular location to another;however, traditional biochemical techniques cannot provide sufficient spatio-temporal information to elucidate these molecular interactions in living cells. Over the past decade, several new techniques have enabled the identification and characterization of PPIs. In this review, we summarize three main techniques for detecting PPIs in vivo, focusing on their basic principles and applications in biological studies. We place a special emphasis on their advantages and limitations, and, in particular, we introduced some uncommon new techniques, such as single-molecule FRET(smFRET), FRET-fluorescence lifetime imaging microscopy(FRET-FLIM), cytoskeleton-based assay for protein-protein interaction(CAPPI) and single-molecule protein proximity index(smPPI), highlighting recent improvements to the established techniques. We hope that this review will provide a valuable reference to enable researchers to select the most appropriate technique for detecting PPIs.

著录项

  • 来源
    《中国科学》 |2019年第5期|P.619-632|共14页
  • 作者单位

    [1]Beijing Advanced Innovation Center for Tree Breeding by Molecular Design;

    Beijing Forestry University;

    Beijing 100083;

    China;

    [2]College of Biological Sciences&Biotechnology;

    Beijing Forestry University;

    Beijing 100083;

    China;

    [1]Beijing Advanced Innovation Center for Tree Breeding by Molecular Design;

    Beijing Forestry University;

    Beijing 100083;

    China;

    [2]College of Biological Sciences&Biotechnology;

    Beijing Forestry University;

    Beijing 100083;

    China;

    [1]Beijing Advanced Innovation Center for Tree Breeding by Molecular Design;

    Beijing Forestry University;

    Beijing 100083;

    China;

    [2]College of Biological Sciences&Biotechnology;

    Beijing Forestry University;

    Beijing 100083;

    China;

    [3]Institute of Plant Stress Biology;

    State Key Laboratory of Cotton Biology;

    Department of Biology;

    Henan University;

    Jinming Street;

    Kaifeng 475001;

    China;

    [3]Institute of Plant Stress Biology;

    State Key Laboratory of Cotton Biology;

    Department of Biology;

    Henan University;

    Jinming Street;

    Kaifeng 475001;

    China;

    [1]Beijing Advanced Innovation Center for Tree Breeding by Molecular Design;

    Beijing Forestry University;

    Beijing 100083;

    China;

    [2]College of Biological Sciences&Biotechnology;

    Beijing Forestry University;

    Beijing 100083;

    China;

  • 原文格式 PDF
  • 正文语种 CHI
  • 中图分类 蛋白质;
  • 关键词

    protein-protein interactions; in vivo; techniques; RET; PCA; co-localization;

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