Objective The purpose of this study is to explore the influence of dihydrotestosterone (DHT) on human endothelial progenitor cells (EPCs) proliferation in vitro.Methods Peripheral blood mononuclear cells were isolated from adult healthy male blood donors.The cells were plated on fibronectin-coated plates and maintained in endothelial growth medium-2 (EGM-2).After 7 days,EPCs were identified by double-positive for DiI AcLDL and FITC-UEA-Ⅰ under laser scanning confocal microscope (LSCM).In order to study the effect of DHT on EPCs,the cells were incubated with a series of concentrations (0,1,10 and100 nMol/L) of DHT for 24 h.The EPCs proliferation was detected by cell counting and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2h-tetrazolium bromide (MTT) assay.Results DHT significantly enhanced the proliferative ability of EPCs with DHT concentration increased (P＜0.01).However,as the amount of DHT attained 100 nMol/L,proliferative ability of EPCs decreased to a level which was still significantly higher than that of control group.Conclusion DHT can enhance proliferation ability of EPCs with a dose-dependent manner in certain limits in vitro.%目的 探讨双氢睾酮(DHT)对人外周血内皮祖细胞增殖能力的影响.方法 采用密度梯度离心法从健康成年男性外周血获得单个核细胞,接种于人纤维连接蛋白包被的培养板.培养7d后,经Dil标记的乙酰化低密度脂蛋白(Dil-acLDL)及FITC标记的荆豆凝集素-Ⅰ(FITC-UEA-Ⅰ)荧光双染法鉴定正在分化的内皮祖细胞.采用人工计数法和3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)法观察浓度为0 nmol/L(对照组),1nmol/L DHT组,10nmol/L DHT组,100 nmol/L DHT组DHT对内皮祖细胞增殖能力的影响.结果 与对照组相比,各浓度DHT组内皮祖细胞增殖能力明显增强,差异有统计学意义(P＜0.01)；随着DHT浓度的升高促进作用逐渐增强,但浓度达100 nmol/L时DHT对内皮祖细胞增殖能力促进作用有所减弱,但仍明显高于对照组,差异有统计学意义(P＜0.01).结论 在一定浓度范围内,DHT可呈剂量依赖性增强内皮祖细胞增殖能力.
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