A method to determine α- spinach sterols and bean-7-ol contents in silver Bupleurum was established. TLC scanning method was used to determine α- spinach sterols and bean-7-ol contents in silver Bupleurum by using 525 nm as determining wavelength and 700 nm as reference wavelength, the mixtureof α- spinasterol (α-spinasterol) and bean-7-ol (stigmast-7-enol) (1: 3) as reference sample. The results show that, α- spinach sterols and bean-7-ol content in 21.46~107.30 μg/μL show a good linear relationship (r= 0.991 1), the average recovery is 99.5% (RSD = 4.8%). The method is simple, time-saving, small loss, and can be used to determine the content of α- spinach sterols and beans-7-enol in silver Bupleurum.%建立银柴胡中α-菠甾醇和豆甾-7-烯醇含量测定的方法。采用薄层扫描法，以525 nm为测定波长，700 nm为参比波长，α-菠甾醇（α-spinasterol）和豆甾-7-烯醇（stigmast-7-enol）的混合物（1：3）为对照品，测定银柴胡中α-菠甾醇和豆甾-7-烯醇的含量。α-菠甾醇和豆甾-7-烯醇含量在21.46～107.30μg/μL 范围内呈良好的线性关系（r=0.9911），平均回收率为99.5%(RSD=4.8%)。结论该方法简单、省时、损失小，可用于测定银柴胡中α-菠甾醇和豆甾-7-烯醇的含量。
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