The mechanisms of antagonists against fungal pathogens have become increasingly important in biological control in recent decades. In this paper, the mechanisms of antagonism, competition, and systemic-resistance induction in the bacterium strain C-02 against Verticillium wilt of cotton were studied by double culture, liquid fermentation, and inducing cotton seedlings, respectively. The results indicated that an antifungal protein from strain C-02 could destroy the cell wall of pathogen strain VD-11 and inhibit its toxin secretion. The results also showed that strain C-02 could colonize the rhizospheric soil of cotton fields better than VD-11, and they had the advantage in carbon resource use. When cotton leaves were treated with a bacterial culture of C-02, PAL, POD and PPO activity was quickly enhanced and remained at high levels for a long time. In conclusion, the antagonistic mechanisms of C-02 against Verticillium wilt of cotton form a comprehensive behavior including antagonism, bacteriolysis, competition, and systemic-resistance induction.%从抗生、竞争和诱导系统抗性三个方面对拮抗细菌C-02防治棉花黄萎病的机理展开了研究,结果表明,C-02分泌的抗菌蛋白能溶解病原菌VD-11的细胞壁,抑制其致萎毒素的分泌.与病原菌VD-11相比,C-02利用碳源有明显优势,在棉花根际土壤中具有显著的营养竞争优势和很好的定殖能力.C-02处理棉苗后,棉叶中植株防御性酶系PAL、POD和PPO的活性提高幅度大,维持时间长.C-02防治棉花黄萎病的机理是抗生、溶菌、竞争和诱导棉苗系统抗性的综合表现.
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