De novo transcriptome assembly of Aureobasidium melanogenum CGMCC18996 to analyze theβ-poly(L-malic acid)biosynthesis pathway under the CaCO_(3) addition

Genan Wang; Haisong Yin; Tingbin Zhao; Donglin Yang; Shiru Jia; Changsheng Qiao

首页> 中文期刊> 《食品科学与人类健康（英文）》 >De novo transcriptome assembly of Aureobasidium melanogenum CGMCC18996 to analyze theβ-poly(L-malic acid)biosynthesis pathway under the CaCO_(3) addition

De novo transcriptome assembly of Aureobasidium melanogenum CGMCC18996 to analyze theβ-poly(L-malic acid)biosynthesis pathway under the CaCO_(3) addition

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β-Poly(L-malic acid)(PMLA)is a water-soluble biopolymer used in food,medicine and other industries.To date,the biosynthesis pathway of PMLA has not been fully elucidated.In this study,we sequenced the transcriptom e of strain Aureobasidium melanogenum under 20 g/L CaCO_(3) addition.The resulting sequencing reads were assembled and annotated for the differentially expressed genes(DEGs)analysis and novel transcripts identification.The result indicated that with the CaCO_(3) addition,the tricarboxylic cycle(TCA)cycle and glyoxylate pathway were up-regulated,and it also found that a non-ribosomal peptide synthetase(NRPS)like protein was highly expressed.The DEGs analysis showed a high expression level of malate dehydrogenase(MDHC)and phosphoenolpyruvate carboxykinase(PCKA)in the CaCO_(3) group,which indicated a cytosolic malate activity.We speculated that the malate should be transported to or synthesized in the cytoplasm,which was then polymerized to PMLA by the NRPS-like protein,accompanied by the up-regulated TCA cycle providing ATP for the polymerization.Depending on the analysis,we assumed that an NRPS-like protein,the TCA cycle,and the cytosolic malate together are contributing to the PMLA biosynthesis.

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《食品科学与人类健康（英文）》 |2023年第4期|1248-1256|共9页
作者
Genan Wang; Haisong Yin; Tingbin Zhao; Donglin Yang; Shiru Jia; Changsheng Qiao;
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作者单位

Key Laboratory of Industrial Fermentation Microbiology(Tianjin University of Science and Technology) of Education 300457;

Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control of Biotechnology University of Science and Technology 300457;

School of Bioengineering Modern Vocational Technology College 300350;

Tianjin Huizhi Biotrans Bioengineering Co.. 300457;

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原文格式 PDF
正文语种 chi
中图分类高分子化学（高聚物）;
关键词
De novo transcriptome analysis; β-Poly(L-malic acid); Aureobasidium melanogenum;

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De novo transcriptome assembly of Aureobasidium melanogenum CGMCC18996 to analyze theβ-poly(L-malic acid)biosynthesis pathway under the CaCO_(3) addition

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