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首页> 中文期刊> 《抗癌研究》 >Curcumin Inhibits Cell Viability by Inducing Apoptosis and Autophagy in Human Colon Cancer Cells

Curcumin Inhibits Cell Viability by Inducing Apoptosis and Autophagy in Human Colon Cancer Cells

         
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摘要

Objective:To explore the inhibitory effect of curcumin on colon cancer in vitro.Methods:Human colon cancer cell lines HT29 and SW620 were cultured in vitro,which were divided into test group and control group.Cells in the test groups were treated with curcumin at different concentrations,while the control groups were treated with dimethylsulfoxide(DMSO).Subsequently,methyl thiazolyl tetrazolium(MTT)assay,were carried out to estimate cellular proliferative activity.Flow cytometry using annexin-V/propidium iodide(PI)staining was performed to detect cell apoptosis.Formation of autophagosomes in the cytoplasm in colon cancer cells were detected by TEM(transmission electron microscopy analysis),and Western blot to measure the expressions of proteins related to apoptosis and autophagy.Results:MTT assay showed that curcumin inhibited the proliferative activity of both HT29 and SW620 cells,significantly different from the control group(P<0.001).The halfmaximal inhibitory concentration(IC50)of curcumin at 24 hours was 20μM for both HT29 and SW620 cells.Annexin-V FITC/PI double labeling showed that,After treatment with curcumin of 20μM for 24 hours,a significant increase was observed in apoptosis rate in both HT219 cells((10.00±0.60)%vs.(4.00±0.70)%,P<0.01;(10.50±0.40)%vs.(4.30±0.26)%,P<0.01)compared with the control cells.TEM analysis showed that curcumin increased the accumulation of autophagosomes in the cytoplasm in HT29 and SW620 cells.Western blot showed an increase in the expressions of apoptosis-related proteins including cleaved caspase-3 and cleaved poly ADP-ribose polymerase(PARP)but decrease of P62 protein in both HT29 and SW620 cells after 24-hour treatment with 20μM curcumin for 24 hours compared with the control groups.Conclusion:Curcumin can inhibit the proliferation,induce apoptosis and autophagy in human colon cancer cells.

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  • 来源
    《抗癌研究》 |2019年第2期|P.21-25|共5页
  • 作者

    Xiaodong Han; Lanjie Guo; Xiaojia Jiang; Yongxin Wang; Zhigang Wang; Dongmei Li;

  • 作者单位

    Department of Gastroenterology Cangzhou People’s Hospital Cangzhou 061000 China;

    Department of Gastroenterology Cangzhou People’s Hospital Cangzhou 061000 China;

    Department of Gastroenterology Cangzhou People’s Hospital Cangzhou 061000 China;

    Cangzhou Medical College Cangzhou 061001 China;

    Department of Gastroenterology Cangzhou People’s Hospital Cangzhou 061000 China;

    Endoscopy Center Cangzhou People’s Hospital Cangzhou 061000 China;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 肿瘤学;
  • 关键词

    Colon cancer; Curcumin; Apoptosis; Autophagy;

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